• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of breath research >Characterization of exhaled breath particles collected by an electret filter technique
【24h】

Characterization of exhaled breath particles collected by an electret filter technique

机译：驻极体过滤器技术收集的呼出气颗粒的特征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aerosol particles that are present in exhaled breath carry nonvolatile components and have gained interest as a specimen for potential biomarkers. Nonvolatile compounds detected in exhaled breath include both endogenous and exogenous compounds. The aim of this study was to study particles collected with a new, simple and convenient filter technique. Samples of breath were collected from healthy volunteers from approximately 30 l of exhaled air. Particles were counted with an optical particle counter and two phosphatidylcholines were measured by liquid chromatography-tandem mass spectrometry. In addition, phosphatidylcholines and methadone was analysed in breath from patients in treatment with methadone and oral fluid was collected with the Quantisal device. The results demonstrated that the majority of particles are <1 mu m in size and that the fraction of larger particle contributes most to the total mass. The phosphatidylcholine PC(16 : 0/16 : 0) dominated over PC(16 : 0/18 : 1) and represented a major constituent of the particles. The concentration of the PC(16 : 0/16 : 0) homolog was significantly correlated (p < 0.001) with total mass. From the low concentration of the two phosphatidylcholines and their relative abundance in oral fluid a major contribution from the oral cavity could be ruled out. The concentration of PC(16 : 0/16 : 0) in breath was positively correlated with age (p < 0.01). An attempt to use PC(16 : 0/16 : 0) as a sample size indicator for methadone was not successful, as the large intra-individual variability between samplings even increased after normalization. In conclusion, it was demonstrated that exhaled breath sampled with the filter device represents a specimen corresponding to surfactant. The possible use of PC(16 : 0/16 : 0) as a sample size indicator was supported and deserves further investigations. We propose that the direct and selective collection of the breath aerosol particles is a promising strategy for measurement of nonvolatiles in breath.
机译：呼气中存在的气溶胶颗粒带有非挥发性成分，作为潜在生物标志物的标本已引起人们的兴趣。呼出气中检测到的非挥发性化合物包括内源性化合物和外源性化合物。这项研究的目的是研究使用新的，简单和方便的过滤器技术收集的颗粒。从大约30升呼出的空气中收集健康志愿者的呼吸样本。用光学颗粒计数器对颗粒计数，并通过液相色谱-串联质谱法测量两个磷脂酰胆碱。此外，对接受美沙酮治疗的患者的呼吸中的磷脂酰胆碱和美沙酮进行了分析，并使用Quantisal装置收集了口服液。结果表明，大多数颗粒的尺寸小于1微米，较大颗粒的部分对总质量的贡献最大。磷脂酰胆碱PC（16：0/16：0）在PC（16：0/18：1）上占主导地位，代表了颗粒的主要成分。 PC（16：0/16：0）同源物的浓度与总质量显着相关（p <0.001）。从两种磷脂酰胆碱的低浓度及其在口腔液中的相对丰度，可以排除口腔的主要作用。呼吸中PC（16：0/16：0）的浓度与年龄呈正相关（p <0.01）。尝试将PC（16：0/16：0）用作美沙酮的样本量指标未成功，因为标准化后样本之间的较大个体内差异甚至增加了。总之，证明了用过滤器装置采样的呼气代表与表面活性剂相对应的样品。支持将PC（16：0/16：0）用作样本量指标，值得进一步研究。我们建议直接和选择性地收集呼吸气溶胶颗粒是测量呼吸中非挥发性物质的一种有前途的策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号