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首页> 外文期刊>Journal of cardiac surgery. >Enterovirus RNA replication in cases of dilated cardiomyopathy: light microscopic in situ hybridization and virological analyses of myocardial specimens obtained at partial left ventriculectomy.
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Enterovirus RNA replication in cases of dilated cardiomyopathy: light microscopic in situ hybridization and virological analyses of myocardial specimens obtained at partial left ventriculectomy.

机译：扩张型心肌病病例中的肠病毒RNA复制：局部左心室切除术获得的心肌标本的光学显微镜原位杂交和病毒学分析。

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OBJECTIVE: Recently, attention has been focused on enteroviral infection of the heart in the genesis of dilated cardiomyopathy (DCM). To determine the location of enteroviral RNA in the myocardium, we performed light microscopic in situ hybridization (ISH) and virological analyses of myocardial specimens obtained at partial left ventriculectomy (PLV). METHODS: Posterolateral walls of the left ventricle from 26 DCM patients were examined. Myocardial specimens were tested for the presence of enteroviral genomes by polymerase chain reaction (PCR). We selected two age-matched groups (10 patients each) in which enteroviruses were either present (EV-plus group) or not (EV-minus group). For both groups, we examined in situ localization of enteroviral RNA in the myocardium by ISH. RESULTS: In PCR studies, both sense and antisense enteroviral RNA were detected in the myocardium of seven patients in the EV-plus group. The presence of this RNA indicates active viral replication in the myocardium. Five of seven patients who exhibited both sense and antisense enteroviral RNA died early after surgery. On ISH, three patients had evidence of active replication of enteroviral genomes. Viral genomes were present in myocardial lesions, especially in endocardial sites. Viral signals were found in degenerating myocardial cells, interstitial inflammatory cells, and endothelial cells of small vessels. These positive signals were not detected in the myocardium of the EV-negative group. CONCLUSIONS: We detected both sense and antisense enteroviral RNA in various myocardial lesions. This suggests that active enteroviral replication plays a role in the development of myocardial lesions in DCM patients. Active viral replication appears to be a prognostic factor for DCM after PLV. Further study of active viral replication in myocardial lesions will provide information useful for evaluating different therapeutic strategies for DCM.
机译：目的：最近，在扩张型心肌病（DCM）的起源中，注意力已集中在心脏的肠病毒感染上。为了确定肠病毒RNA在心肌中的位置，我们进行了光学显微原位杂交（ISH）和在部分左心室切除术（PLV）中获得的心肌标本的病毒学分析。方法：检查了26名DCM患者的左心室后外侧壁。通过聚合酶链反应（PCR）检测心肌标本中是否存在肠道病毒基因组。我们选择了两个年龄匹配的组（每组10例），其中存在或不存在（EV加组）（EV减组）的肠病毒。对于这两组，我们通过ISH检查了心肌中肠病毒RNA的原位定位。结果：在PCR研究中，EV加组的7例患者的心肌中均检测到有义和反义肠病毒RNA。该RNA的存在表明心肌中病毒活跃复制。表现出正义和反义肠病毒RNA的七名患者中有五名在手术后早期死亡。在ISH上，三名患者有肠病毒基因组活跃复制的证据。病毒基因组存在于心肌病变中，尤其是在心内膜部位。在退化的心肌细胞，间质性炎症细胞和小血管内皮细胞中发现了病毒信号。在EV阴性组的心肌中未检测到这些阳性信号。结论：我们在各种心肌损伤中都检测到有义和反义肠病毒RNA。这表明主动肠病毒复制在DCM患者心肌病变的发展中起作用。主动病毒复制似乎是PLV后DCM的预后因素。心肌损伤中病毒复制的进一步研究将为评估DCM的不同治疗策略提供有用的信息。

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