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首页> 外文期刊>Journal of cardiovascular electrophysiology >Behavior of nonselective cation channels and large-conductance Ca2+-activated K+ channels induced by dynamic changes in membrane stretch in cultured smooth muscle cells of human coronary artery.
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Behavior of nonselective cation channels and large-conductance Ca2+-activated K+ channels induced by dynamic changes in membrane stretch in cultured smooth muscle cells of human coronary artery.

机译：在人冠状动脉培养的平滑肌细胞中，由膜拉伸动态变化引起的非选择性阳离子通道和大电导Ca2 +激活的K +通道的行为。

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INTRODUCTION: The effects of membrane stretch on ion channels were investigated in cultured smooth muscle cells of human coronary artery. METHODS AND RESULTS: In the cell-attached configuration, membrane stretch with negative pressure induced two types of stretch-activated (SA) ion channels: a nonselective cation channel and a large-conductance Ca2+-activated K+ (BK(Ca)) channel. The single-channel conductances of SA cation and BK(Ca) channels were 26 and 203 pS, respectively. To elucidate the mechanism of activation of these SA channels and to minimize mechanical disruption, a sinusoidal change in pipette pressure was applied to the on-cell membrane patch. During dynamic changes in pipette pressure, increases in SA cation channel activity was found to coincide with increases in BK(Ca) channel activity. In the continued presence of cyclic stretch, the activity of SA cation channels gradually diminished. However, after termination of cyclic stretch, BK(Ca) channel activity was greatly enhanced, but the activity of SA cation channels disappeared. CONCLUSION: This study is the first to demonstrate that the behavior of SA cation and BK(Ca) channels in coronary smooth muscle cells is differentially susceptible to dynamic changes in membrane tension.
机译：简介：在人冠状动脉培养的平滑肌细胞中研究了膜拉伸对离子通道的影响。方法和结果：在细胞附着的配置中，带有负压的膜拉伸诱导了两种类型的拉伸激活（SA）离子通道：非选择性阳离子通道和大电导Ca2 +激活的K +（BK（Ca））通道。 SA阳离子和BK（Ca）通道的单通道电导分别为26和203 pS。为了阐明这些SA通道的激活机制并最大程度地减少机械干扰，将吸管压力的正弦变化应用于细胞上的膜片。在移液器压力的动态变化过程中，发现SA阳离子通道活性的增加与BK（Ca）通道活性的增加相一致。在持续存在循环拉伸的情况下，SA阳离子通道的活性逐渐降低。但是，循环拉伸终止后，BK（Ca）通道的活性大大增强，但SA阳离子通道的活性却消失了。结论：本研究首次证明冠状动脉平滑肌细胞中SA阳离子和BK（Ca）通道的行为对膜张力的动态变化具有不同的敏感性。

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