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首页> 外文期刊>Journal of Cereal Science >Evaluation of thermomechanical pretreatment for enzymatic hydrolysis of pure microcrystalline cellulose and cellulose from Brewers' spent grain.
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Evaluation of thermomechanical pretreatment for enzymatic hydrolysis of pure microcrystalline cellulose and cellulose from Brewers' spent grain.

机译：评估酶促水解纯微晶纤维素和布鲁尔废谷物中纤维素的热机械预处理。

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摘要

Microcrystalline cellulose (Avicel PH102) and Brewers' spent grain (BSG) were subjected to Detente Instantanee Controlee (DIC) thermomechanical pre-treatment before exposure to cellulases (Celluclast 1.5 L). In a first part, we showed that the addition of beta-glucosidase (Novozym-188) increased the hydrolysis yield of Avicel. A maximal theoretical yield (100%), was obtained for 5 and 10 g/l of Avicel using a mixture of Celluclast 1.5 l/Novozym-188. After DIC pre-treatments, the initial rate and final yield of hydrolysis decreased in comparison with those from untreated microcrystalline cellulose. This phenomenon may be due to the modification of the crystallinity of pure cellulose and the formation of inhibitors during the pre-treatment. In a second part, BSG was thermomechanically pre-treated and hydrolyzed. The results showed that the hydrolysis yield of BSG treated at pressure levels between 2 and 7 bar during 15 min was strongly improved compared to hydrolysis yield of untreated BSG. The optimized hydrolysis process, under intensive DIC conditions, achieved a glucose yield corresponding to 100% of the theoretical cellulose value. The morphology of BSG samples was studied with Scanning Electronic Microscopy (SEM) and highlighted that the structure of pre-treated BSG showed an important disruption compared to the rigid structure of untreated BSG
机译：在暴露于纤维素酶（Celluclast 1.5 L）之前，对微晶纤维素（Avicel PH102）和Brewers的废谷粒（BSG）进行了缓和瞬时控制（DIC）热机械预处理。在第一部分中，我们表明添加β-葡萄糖苷酶（Novozym-188）可提高Avicel的水解产量。使用1.5 l / Novozym-188 Celluclast的混合物获得5和10 g / l Avicel的最大理论产率（100％）。与未处理的微晶纤维素相比，经过DIC预处理后，水解的初始速率和最终产率降低。这种现象可能是由于在预处理过程中纯纤维素结晶度的改变和抑制剂的形成所致。在第二部分中，对BSG进行了热机械预处理和水解。结果表明，与未处理的BSG的水解产率相比，在2-7 bar的压力水平下处理15分钟的BSG的水解产率得到了极大的提高。在强化DIC条件下，经过优化的水解过程实现了相当于理论纤维素值100％的葡萄糖产率。使用扫描电子显微镜（SEM）研究了BSG样品的形貌，并强调与未处理的BSG的刚性结构相比，预处理的BSG的结构显示出重要的破坏性。

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