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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Immobilization of beta-amylase using polyacrylamide polymer derivatives
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Immobilization of beta-amylase using polyacrylamide polymer derivatives

机译：使用聚丙烯酰胺聚合物衍生物固定β-淀粉酶

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摘要

Barley beta-amlyase was immobilized on two polymeric materials; poly(acrylamide-acrylic acid) resin [P(AM-AAc)] and poly(acrylamide-acrylic acid-diallylamine-HCl) resin [P(P(AM-AAc-DAA-HCl) using two different methods: covalent and cross-linking immobilization. Thionyl chloride, used to activate the polymers for covalent immobilization, has the advantage that it is able to react with a number of surface groups of protein under very mild conditions. Cross-linking with glutaraldehyde gave a higher coupling yield (approximately 70%) than covalent immobilization (approximately 20%). The activity and stability of the resulting biopolymers have been compared with those of free beta-amylase. The specific activity of the immobilized enzyme was significantly influenced by the amount of enzyme loaded onto the polymers, the optimal level being 3.5 mg g(-1) polymer. It was found that the immobilized beta-amylase stored at VC retained approximately 90% of its original activity after 30 days, whereas free beta-amylase stored in solution at 4degreesC retained only 47% of its activity after same period. The difference in long term stability was more significant when the enzyme was stored at room temperature; the immobilized enzyme maintained 40% of its activity after 30 days, whereas the residual activity of free enzyme was only 10%. (C) 2003 Society of Chemical Industry.
机译：大麦β-淀粉酶固定在两种聚合材料上;聚（丙烯酰胺-丙烯酸）树脂[P（AM-AAc）]和聚（丙烯酰胺-丙烯酸-二烯丙胺-HCl）树脂[P（P（AM-AAc-DAA-HCl）]使用共价和交叉两种方法固定化：亚硫酰氯用于活化聚合物以进行共价固定，其优点是能够在非常温和的条件下与蛋白质的许多表面基团反应。与戊二醛的交联具有更高的偶联收率（大约）比共价固定化（约20％）高70％），将所得生物聚合物的活性和稳定性与游离β-淀粉酶进行了比较，固定化酶的比活度受负载在聚合物上的酶量的显着影响最佳水平为3.5 mg g（-1）聚合物，发现在VC中储存的固定化β-淀粉酶在30天后保留了其原始活性的90％，而在4°C的溶液中储存的游离β-淀粉酶仅保留了y同期后其活动的47％。当酶在室温下保存时，长期稳定性的差异更为明显。固定化酶在30天后保持其活性的40％，而游离酶的残留活性仅为10％。 （C）2003年化学工业协会。

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