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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Lactic acid fermentation of food waste using integrated glucoamylase production
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Lactic acid fermentation of food waste using integrated glucoamylase production

机译：利用整合的葡糖淀粉酶生产食物垃圾的乳酸发酵

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摘要

Commercial enzyme is usually needed for the bioconversion of organic waste or biomass. The overall cost could be reduced very significantly if enzyme production could be integrated with its application, avoiding unnecessary steps in enzyme production (such as concentration, recovery and transportation). This investigation attempted to integrate crude glucoamylase production with lactic acid fermentation of food waste. A maximum glucoamylase activity of 1850 U g' was obtained with Aspergillus niger during solid-state fermentation (SSF) of food waste, 14.8 times more than that obtained during submerged fermentation (SmF). The optimum pH for producing glucoamylase was 4.6, and glucoamylase retained 83.5% of peak activity at pH 3.0. Without any recovery treatment, the glucoamylase produced by SSF could be used directly for lactic acid fermentation of food waste. Lactic acid concentration reached 45.5 g L~(-1) with the addition of the crude enzyme, 72% higher than the control. No side-effects were caused by the viable A. niger in the crude enzyme. This work successfully integrated glucoamylase production with lactic acid fermentation. The enzyme produced by 55F of food waste had sufficient activity to be used directly without any treatment. The integrated process proposed in this study was very economical and may be helpful to other bioconversions.
机译：有机废物或生物质的生物转化通常需要商业酶。如果可以将酶的生产与其应用相结合，则可以大大降低总成本，从而避免了酶生产中不必要的步骤（例如浓缩，回收和运输）。这项研究试图将粗葡糖淀粉酶的生产与餐厨垃圾的乳酸发酵相结合。在食物垃圾的固态发酵（SSF）期间，黑曲霉获得的最大葡糖淀粉酶活性为1850 U g'，是深层发酵（SmF）的14.8倍。用于产生葡糖淀粉酶的最佳pH为4.6，并且葡糖淀粉酶在pH 3.0下保留了峰值活性的83.5％。无需任何回收处理，SSF生产的葡糖淀粉酶可直接用于食品垃圾的乳酸发酵。加入粗酶后，乳酸浓度达到45.5 g L〜（-1），比对照高72％。粗酶中活的黑曲霉没有引起任何副作用。这项工作成功地将葡糖淀粉酶生产与乳酸发酵整合在一起。 55F厨余产生的酶具有足够的活性，无需任何处理即可直接使用。这项研究中提出的整合过程非常经济，可能有助于其他生物转化。

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