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首页> 外文期刊>Journal of chemical neuroanatomy >Hippocampal CA field neurogenesis after pilocarpine insult: The hippocampal fissure as a neurogenic niche
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Hippocampal CA field neurogenesis after pilocarpine insult: The hippocampal fissure as a neurogenic niche

机译：毛果芸香碱损伤后海马CA场神经发生：海马裂隙为神经源性利基

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Pilocarpine model for temporal lobe epilepsy has shown aberrant neurogenesis, but mainly restricted to the dentate gyrus (DG). Herein, by using a modified protocol, combining pilocarpine with ipratropium bromide, we unexpectedly observed a heretofore-unrecognized distinct cellular population expressing the neuroprogenitor marker doublecortin (DCX) on post insult days (PID) 10,14 and 18, mainly located in the temporal segment of the hippocampal fissure (hf). Some of these DCX+ cells possessed high morphological complexity and seemed to disperse toward the CA fields. Next, we injected bromodeoxyuridine (BrdU) in early (PID 2-4) and delayed (PID 5-7) fashions and killed the rats 7-35 days later for immunohistochemical and anatomical analysis. Massive increase of BrdU labeling was found in the delayed group and the neural stem cell-specific marker nestin was highly expressed in the same narrow band on PID7, so was glial fibrillary acidic protein (GFAP). Using double labeling with BrdU and a mature neuron marker NeuN, we found discrete but clear BrdU+/NeuN+ double labeled cells in the Cornu Ammonis (CA) pyramidal cell layer on PID35. Based on immunohistochemical and anatomical observations, as well as time-course analysis of BrdU, nestin, GFAP, DCX and NeuN expressions in this population of cells located inear hf, we wish to suggest that this structure harbors neurogenic niches, in addition of the possible dispersion of neuroprogenitors from subgranular niches to CA fields also revealed by this study. Our results support the few previous reports demonstrating hippocampal CA field neurogenesis in adult rats. Mechanistic basis of the phenomenon is discussed.
机译：颞叶癫痫的毛果芸香碱模型显示出异常的神经发生，但主要限于齿状回（DG）。在这里，通过使用修改后的协议，结合毛果芸香碱和异丙托溴铵，我们出乎意料地观察到迄今无法识别的不同的细胞群体，其在损伤后天（PID）10、14和18时表达神经祖细胞标记双皮质素（DCX），主要位于颞侧。海马裂孔（hf）段。这些DCX +细胞中有些具有较高的形态复杂性，并且似乎向CA领域扩散。接下来，我们以早期方式（PID 2-4）和延迟方式（PID 5-7）注射了溴脱氧尿苷（BrdU），并在7-35天后杀死了大鼠进行免疫组织化学和解剖学分析。延迟组发现BrdU标记大量增加，神经干细胞特异性标志物Nestin在PID7的同一窄带中高表达，神经胶质纤维酸性蛋白（GFAP）也是如此。使用BrdU和成熟的神经元标记NeuN双重标记，我们在PID35的Cornu Ammonis（CA）锥体细胞层中发现了离散但清晰的BrdU + / NeuN +双重标记细胞。根据免疫组织化学和解剖学观察，以及在位于hf / hf附近的该细胞群中BrdU，nestin，GFAP，DCX和NeuN表达的时程分析，我们希望表明该结构还具有神经源性壁ni。这项研究还揭示了神经祖细胞可能从颗粒下的壁ni扩散到CA区域。我们的研究结果支持了以前的一些研究，这些研究表明成年大鼠海马CA场神经发生。讨论了该现象的机理基础。

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