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首页> 外文期刊>Clinical Biochemistry >Kinetic studies of the interaction between antitumor antibiotics and DNA using quartz crystal microbalance.
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Kinetic studies of the interaction between antitumor antibiotics and DNA using quartz crystal microbalance.

机译：使用石英晶体微量天平进行抗肿瘤抗生素与DNA相互作用的动力学研究。

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OBJECTIVE: Kinetic studies of the interaction process between antitumor antibiotics, Mitomycin C (MMC) and Bleomycin (BLM), and DNA were performed with a novel analytical method, piezoelectric quartz crystal (PQC) impedance analysis. DESIGN AND METHODS: DNA was directly immobilized on the Au-electrode surface of a piezoelectric quartz crystal by adsorption. The DNA-modified piezoelectric sensor was in contact with Mitomycin C and Bleomycin solution, respectively. RESULTS: The experimental results demonstrated that antitumor antibiotics concentration had an effect on the interaction. A pseudo-first-order kinetic model for the interaction between antitumor antibiotics and DNA was derived to describe the process. All fitted results were well in agreement with the corresponding experimental results. The kinetic parameters, the interaction rate constants (k(MMC) and k(BLM)), were determined by fitting experimental data to the model. At 37 +/- 0.5 degrees C, the k(MMC) and k(BLM) values obtained were 4.56 (+/-0.02) x 10(-3) and 9.11 (+/-0.02) mM(-1) s(-1), respectively. CONCLUSION: Piezoelectric quartz crystal impedance (PQCI) analysis is a very powerful method to study the kinetic process of antitumor drugs and DNA interaction.
机译：目的：采用新型分析方法，压电石英晶体（PQC）阻抗分析，对抗肿瘤抗生素丝裂霉素C（MMC）和博来霉素（BLM）与DNA之间的相互作用过程进行动力学研究。设计与方法：通过吸附将DNA直接固定在压电石英晶体的Au电极表面上。 DNA修饰的压电传感器分别与丝裂霉素C和博来霉素溶液接触。结果：实验结果表明，抗肿瘤抗生素的浓度对相互作用有影响。推导了抗肿瘤抗生素与DNA之间相互作用的伪一级动力学模型来描述这一过程。所有拟合结果均与相应的实验结果完全吻合。通过将实验数据拟合到模型中，确定动力学参数，相互作用速率常数（k（MMC）和k（BLM））。在37 +/- 0.5摄氏度下，获得的k（MMC）和k（BLM）值为4.56（+/- 0.02）x 10（-3）和9.11（+/- 0.02）mM（-1）s（ -1）。结论：压电石英晶体阻抗（PQCI）分析是研究抗肿瘤药物动力学过程和DNA相互作用的一种非常有效的方法。

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