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首页> 外文期刊>Journal of Comparative Pathology >Comparative Respiratory Pathogenicity and Dynamic Tissue Distribution of Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and its Attenuated Strain in Piglets
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Comparative Respiratory Pathogenicity and Dynamic Tissue Distribution of Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and its Attenuated Strain in Piglets

机译：中国猪高致病性猪繁殖与呼吸综合征病毒及其减毒株的比较呼吸致病性和动态组织分布

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摘要

The outbreak of highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) in 2006 devastated the Chinese swine industry. HP-PRRS virus is still the predominant strain in mainland China, rather than the classical PRRSV strain, and the attenuated live vaccine remains the preferred choice for protecting piglets against HP-PRRSV infection. To fully evaluate the safety of strain GDr180, the 180th attenuated virus of the HP-PRRSV strain GD, we used clinicopathological, microscopical, ultrastructural, serological and molecular biological methods to assess the different clinical manifestations and respiratory characteristics of piglets inoculated with HP-PRRSV strain GD or strain GDr180. The 5-week-old piglets inoculated with strain GD displayed marked clinical signs, including fever, anorexia, dyspnoea and tachypnoea. Significant interstitial pneumonia was present, characterized by thickened alveolar septa infiltrated with mononuclear cells and cell debris. However, the piglets inoculated with strain GDr180 and the negative control piglets showed neither clinical signs nor microscopical or ultrastructural lesions. Ultrastructural observation of the piglets' tracheas and examination of the dynamic tissue distributions of PRRSV strain GD and attenuated strain GDr180, by immunohistochemistry and fluorescence quantitative reverse transcription-polymerase chain reaction, confirmed significant differences in their pathogenicity and distribution in the respiratory systems of piglets. The differences in pathogenicity are attributable to the different severity of the pathological changes in the pigs inoculated with the two strains. Thus, the HP-PRRSV GDr180 strain is practically harmless to the respiratory systems of piglets and may be a safe candidate for inducing immunity against HP-PRRS. (C) 2015 Elsevier Ltd. All rights reserved.
机译：2006年，高致病性猪繁殖与呼吸综合征（HP-PRRS）的爆发摧毁了中国养猪业。 HP-PRRS病毒仍是中国大陆的主要毒株，而不是经典的PRRSV毒株，减毒活疫苗仍然是保护仔猪免受HP-PRRSV感染的首选。为了全面评估HP-PRRSV GD株的第180株减毒病毒GDr180的安全性，我们使用临床病理，显微镜，超微结构，血清学和分子生物学方法评估了接种HP-PRRSV的仔猪的不同临床表现和呼吸道特征菌株GD或菌株GDr180。接种GD株的5周龄仔猪表现出明显的临床体征，包括发烧，厌食，呼吸困难和呼吸急促。存在严重的间质性肺炎，其特征是肺泡隔层增厚，浸润有单核细胞和细胞碎片。但是，接种GDr180品系的仔猪和阴性对照仔猪均未显示出临床体征，也未显示出微观或超微结构损伤。通过免疫组织化学和荧光定量逆转录-聚合酶链反应对仔猪气管的超微结构观察以及PRRSV菌株GD和减毒菌株GDr180的动态组织分布检查，证实了仔猪在呼吸系统中的致病性和分布存在显着差异。致病性的差异可归因于用两种菌株接种的猪的病理变化程度不同。因此，HP-PRRSV GDr180菌株实际上对仔猪的呼吸系统无害，并且可能是诱导针对HP-PRRS免疫的安全候选者。 （C）2015 Elsevier Ltd.保留所有权利。

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