• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical Biochemistry >Preparation and quantification of methylglyoxal in human plasma using reverse-phase high-performance liquid chromatography.
【24h】

Preparation and quantification of methylglyoxal in human plasma using reverse-phase high-performance liquid chromatography.

机译：使用反相高效液相色谱法制备和定量测定人血浆中的甲基乙二醛。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objectives: To detect methylglyoxal (MG), a highly reactive alpha-oxoaldehyde found widespread throughout biological life, in human plasma using reverse-phase high-performance liquid chromatography method (RP HPLC) with UV detection. Design and methods: The processing of human plasma required protein precipitation with trifluoroacetic acid (TFA), incubation of the supernatant (2 h) with 1,2-diamino-4,5-dimethoxybenzene (DDB) to convert MG to 6,7-dimethoxy-2-methylquinoxaline (DMQ), freeze-drying, and RP HPLC analysis using 6,7-dimethoxy-2,3-dimethylquinoxaline (DMDQ) as an internal standard (IS). Simplified methods for the synthesis of MG and DDB are also described. Results: Calibration curves were linear in the range of 200-1000 nM. The limit of detection was 30.6 and 45.9 pmol, at 215 and 352 nm, respectively. The intraday coefficients of variation were 6.9-12.6% for 215 nm and 3.5-12.6% for 352 nm. The interday coefficients of variation were 9.6-12.8% for 215 nm and 7.2-14.7% for 352 nm. Sample storage conditions together with statistical evaluation are also described. Conclusions: Here we present a rapid and inexpensive method for the determination of methylglyoxal in human plasma using RP HPLC with UV detection. The simplicity of the reported RP HPLC method makes it suitable for the detection of methylglyoxal in many human plasma samples.
机译：目的：使用反相高效液相色谱法（UV反相）检测人类血浆中的甲基乙二醛（MG），这是一种在整个生物生命中广泛发现的高反应性α-氧醛。设计和方法：处理人血浆需要用三氟乙酸（TFA）沉淀蛋白质，将上清液与1,2-二氨基-4,5-二甲氧基苯（DDB）孵育（2小时）以将MG转化为6,7-使用6,7-二甲氧基-2,3-二甲基喹喔啉（DMDQ）作为内标（IS）对二甲氧基-2-甲基喹喔啉（DMQ）进行冷冻干燥和RP HPLC分析。还描述了用于合成MG和DDB的简化方法。结果：校准曲线在200-1000 nM范围内呈线性。在215和352 nm处的检出限分别为30.6和45.9 pmol。 215 nm的日内波动系数为6.9-12.6％，352 nm的日内变异系数为3.5-12.6％。日间变异系数对于215 nm为9.6-12.8％，对于352 nm为7.2-14.7％。还描述了样品存储条件以及统计评估。结论：在这里，我们提出了一种快速且廉价的方法，使用RP HPLC和紫外检测器测定人血浆中的甲基乙二醛。所报道的RP HPLC方法的简便性使其适用于许多人血浆样品中甲基乙二醛的检测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号