...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Comparative Physiology, B. Biochemical, Systemic, and Environmental Physiology >Response of lactate metabolism in brain glucosensing areas of rainbow trout (Oncorhynchus mykiss) to changes in glucose levels
【24h】

Response of lactate metabolism in brain glucosensing areas of rainbow trout (Oncorhynchus mykiss) to changes in glucose levels

机译：虹鳟（Oncorhynchus mykiss）脑葡萄糖摄取区域乳酸代谢对葡萄糖水平变化的响应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is no evidence in fish brain demonstrating the existence of changes in lactate metabolism in response to alterations in glucose levels. We induced in rainbow trout through intraperitoneal (IP) treatments, hypoglycaemic or hyperglycaemic changes to assess the response of parameters involved in lactate metabolism in glucosensing areas like hypothalamus and hindbrain. To distinguish those effects from those induced by peripheral changes in the levels of metabolites or hormones, we also carried out intracerebroventricular (ICV) treatments with 2-deoxy-d-glucose (2-DG, a non-metabolizable glucose analogue thus inducing local glucopenia) or glucose. Finally, we also incubated hypothalamus and hindbrain in vitro in the presence of increased glucose concentrations. The changes in glucose availability were in general correlated to changes in the amount of lactate in both areas. However, when we assessed in these areas the response of parameters related to lactate metabolism, the results obtained were contradictory. The increase in glucose levels did not produce in general the expected changes in those pathways with only a minor increase in their capacity of lactate production. The decrease in glucose levels was, however, more clearly related to a decreased capacity of the pathways involved in the production and use of lactate, and this was especially evident after ICV treatment with 2-DG in both areas. In conclusion, the present results while addressing the existence of changes in lactate metabolism after inducing changes in glucose levels in brain glucosensing areas only partially support the possible existence of an astrocyte-neuron lactate shuttle in hypothalamus and hindbrain of rainbow trout relating glucose availability to lactate production and use.
机译：鱼脑中没有证据表明存在响应葡萄糖水平变化的乳酸代谢变化。我们通过腹膜内（IP）处理，降血糖或高血糖变化诱发虹鳟鱼，以评估涉及下丘脑和后脑的葡萄糖代谢区域的乳酸代谢相关参数的响应。为了将这些效应与周围代谢物或激素水平变化所诱发的效应区分开来，我们还使用2-脱氧-d-葡萄糖（2-DG，一种不可代谢的葡萄糖类似物，从而诱导了局部性低糖血症）进行了脑室内（ICV）治疗。 ）或葡萄糖。最后，我们还在葡萄糖浓度增加的情况下在体外孵育了下丘脑和后脑。葡萄糖可用性的变化通常与两个区域中乳酸含量的变化相关。但是，当我们在这些领域评估与乳酸代谢有关的参数的响应时，获得的结果是矛盾的。葡萄糖水平的增加通常不会在这些途径中产生预期的变化，而它们的乳酸生产能力仅会轻微增加。然而，葡萄糖水平的下降更明显地与乳酸的产生和使用所涉及的途径的能力下降有关，这在两个区域用2-DG进行ICV处理后尤为明显。总而言之，本结果解决了诱导脑葡萄糖代谢区域葡萄糖水平变化后乳酸代谢的变化，仅部分支持下丘脑和虹鳟后脑中星形胶质神经元乳酸穿梭的可能存在，这与葡萄糖的可利用性有关生产和使用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号