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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >An efficient new method for extraction, separation and purification of psoralen and isopsoralen from Fructus Psoraleae by supercritical fluid extraction and high-speed counter-current chromatography
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An efficient new method for extraction, separation and purification of psoralen and isopsoralen from Fructus Psoraleae by supercritical fluid extraction and high-speed counter-current chromatography

机译：一种超临界流体萃取-高速逆流色谱法提取补骨脂中补骨脂素和异补骨脂素的高效新方法

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摘要

Psoralen and isopsoralen were extracted from Fructus Psoraleae (Psoralea corylitolia L.) by supercritical CO2. The effect of various parameters. i.e.. pressure. temperature and sample particle size on yield was investigated with an analytical-scale supercritical fluid extraction (SFE) system to find the optimal conditions. The process was then scaled up by 50 times with a preparative SFE system under the optimized conditions of pressure (26 MPa), temperature (60degreesC) and a sample particle size of 40-60 mesh. The yield of the preparative SFE was 9.1% and the combined yield of psoralen and isopsoralen was 2.5 mg/g of dry seeds. Psoralen and isopsoralen in the extract were separated and purified by high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.7:1:0.8. v/v), and the fractions were analyzed by HPLC, MS, H-1 NMR and C-13 NMR. The structures of the products were further confirmed by comparison with authentic samples (National Institute of the Control of Pharmaceutical and Biological Products, Beijing, China). (C) 2004 Elsevier B.V. All rights reserved.
机译：补骨脂素和异补骨脂素通过超临界CO 2提取自补骨脂（Psoralea corylitolia L.）。各种参数的影响。即压力。用分析规模的超临界流体萃取（SFE）系统研究了温度和样品粒度对产率的影响，以找到最佳条件。然后，在最佳压力，压力（26 MPa），温度（60摄氏度）和样品粒度为40至60目的最佳条件下，使用制备型SFE系统将工艺放大50倍。制备的SFE的产率为9.1％，补骨脂素和异补骨脂素的总产量为2.5 mg / g干种子。分离提取物中的补骨脂素和异补骨脂素，并通过高速逆流色谱法进行纯化，该色谱法采用由正己烷-乙酸乙酯-甲醇-水（1：0.7：1：0.8。v / v）组成的两相溶剂系统，并通过HPLC，MS，H-1 NMR和C-13 NMR分析级分。通过与真实样品进行比较，进一步确定了产品的结构（国家药品和生物产品控制研究所，中国北京）。 （C）2004 Elsevier B.V.保留所有权利。

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