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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection
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Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection

机译:脉冲安培检测法在二氧化钛柱上进行离子色谱法分析植物中的糖磷酸酯

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This paper describes the development of a practical method for the analysis of sugar phosphates from the model higher plant Arabidopsis thaliana by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The extraction method of sugar phosphates from higher plants was first optimized for HPAEC-PAD analysis. In order to improve the resolution in HPAEC-PAD. a column packed with titanium dioxide resin was used. The titanium dioxide Column was used as a trap-column for sugar phosphates and nucleotides, for the removal of sample matrices. Sample pretreatment was achieved in-line and automatically using a six-port valve placed after the injection valve. (C) 2004 Elsevier B.V. All rights reserved.
机译:本文介绍了一种通过高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)分析高等植物拟南芥模型中糖磷酸盐的实用方法的开发。首先优化了高等植物中糖磷酸酯的提取方法,用于HPAEC-PAD分析。为了提高HPAEC-PAD中的分辨率。使用填充有二氧化钛树脂的柱。二氧化钛色谱柱用作糖磷酸盐和核苷酸的捕集柱,用于去除样品基质。在线进行样品预处理,并使用在进样阀之后的六通阀自动进行。 (C)2004 Elsevier B.V.保留所有权利。

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