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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Analysis of cocaine and three of its metabolites in hair by gas chromatography-mass spectrometry using ion-trap detection for CI/MS/MS
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Analysis of cocaine and three of its metabolites in hair by gas chromatography-mass spectrometry using ion-trap detection for CI/MS/MS

机译：气相色谱-质谱联用离子阱检测CI / MS / MS分析头发中的可卡因及其三种代谢物

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摘要

A sensitive GC/CI/MS/MS method was developed for the simultaneous determination of cocaine (COC), anhydroecgonine methylester (cocaine pyrolysis product, AEME), ecgonine methylester (cocaine enzymatic hydrolysis product, EME) and cocaethylene (cocaine with ethanol trans-esterification product, COET) in human hair samples. After acid hydrolysis, hair samples were extracted with an automated solid phase extraction (SPE). The analysis of cocaine and its three metabolites was performed using an ion-trap spectrometer in positive chemical ionization with isobutane as gas reagent. The procedure was validated. Weighted linear regression was found appropriate in a concentration range of 0.10-5.00ng/mg for AEME, 0.05-5.00ng/mg for COC, EME and COET. The limit of detection was estimated at 0.005 ng/mg for COC and COET, at 0.025 ng/mg for EME, and at 0.050 ng/mg for AEME. Method performance was evaluated in terms of trueness and precision using quality control (QC) samples over the investigated ranges. Method selectivity and robustness were also demonstrated. (c) 2005 Elsevier B.V. All rights reserved.
机译：开发了一种灵敏的GC / CI / MS / MS方法，用于同时测定可卡因（COC），脱水电子精氨酸甲酯（可卡因热解产物AEME），电子精氨酸甲酯（可卡因酶促水解产物EME）和可卡乙烯（可卡因与乙醇反式人发样品中的酯化产物（COET）。酸水解后，用自动固相萃取（SPE）萃取头发样品。可卡因及其三种代谢物的分析使用离子阱光谱仪在异丁烷作为气体试剂进行正化学电离的过程中进行。该程序已验证。加权线性回归适用于AEME的浓度范围为0.10-5.00ng / mg，适用于COC，EME和COET的浓度范围为0.05-5.00ng / mg。对于COC和COET，检测极限估计为0.005 ng / mg，对于EME为0.025 ng / mg，对于AEME为0.050 ng / mg。使用研究范围内的质量控制（QC）样品，根据真实性和精密度评估了方法的性能。还证明了方法的选择性和鲁棒性。 （c）2005 Elsevier B.V.保留所有权利。

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