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首页> 外文期刊>Journal of Dental Research: Official Publication of the International Association for Dental Research >Ml80 Amelogenin Processed by MMP20 is Sufficient for Decussating Murine Enamel
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Ml80 Amelogenin Processed by MMP20 is Sufficient for Decussating Murine Enamel

机译：MMP20处理的Ml80釉蛋白足以降解鼠釉

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摘要

Amelogenin (AMELX) and matrix metalloproteinase-20 (MMP20) are essential for proper enamel development. Amelx and Mmp20 mutations cause amelogenesis imper-fecta. MMP20, a protease secreted by ameloblasts, is responsible for processing enamel proteins, including AMELX, during the secretory stage of enamel formation. Of at least 16 different amelogenin splice products, the most abundant isoform found in murine ameloblasts and developing enamel is the Ml80 protein. To understand the role of MMP20 processing of Ml80 AMELX, we generated AmelxKO/Mmp20KO (DKO) mice with an amelogenin (M180Tg) transgene. We analyzed the enamel phenotype by SEM to determine enamel structure and thickness, uCT, and by nanoindentation to quantify enamel mechanical properties. M180Tg/DKO mouse enamel had 37% of the hardness of M180Tg/AmelxKO teeth and demonstrated a complete lack of normal prismatic architecture. Although molar enamel of M180Tg/AmelxKO mice was thinner than WT, it had similar mechanical properties and decussating enamel prisms, which were abolished by the loss of MMP20 in the M180Tg/DKO mice. Retention of the C-terminus or complete lack of this domain is unable to rescue amelogenin null enamel. We conclude that among amelogenins, Ml80 alone is sufficient for normal enamel mechanical properties and prism patterns, but that additional amelogenin splice products are required to restore enamel thickness.
机译：Amelogenin（AMELX）和基质金属蛋白酶20（MMP20）对于正确的牙釉质发育至关重要。 Amelx和Mmp20突变导致釉质生成不全。 MMP20是成釉细胞分泌的一种蛋白酶，在搪瓷形成的分泌阶段负责处理搪瓷蛋白，包括AMELX。在至少16种不同的釉原蛋白剪接产物中，在鼠成釉细胞和发育中的釉质中发现的最丰富的同工型是M180蛋白。为了了解MMP20处理M180 AMELX的作用，我们生成了具有釉原蛋白（M180Tg）转基因的AmelxKO / Mmp20KO（DKO）小鼠。我们通过SEM分析了牙釉质的表型，以确定牙釉质的结构和厚度，uCT，并通过纳米压痕分析了牙釉质的机械性能。 M180Tg / DKO小鼠牙釉质的硬度为M180Tg / AmelxKO牙齿的37％，并且完全缺乏正常的棱柱结构。尽管M180Tg / AmelxKO小鼠的磨牙珐琅质比WT薄，但它具有相似的机械性能和破坏性的釉质棱柱，而M180Tg / DKO小鼠中MMP20的丧失消除了这些缺陷。保留C末端或完全缺乏该结构域无法挽救牙釉蛋白原釉质。我们得出结论，在牙釉蛋白中，单独的Ml80足以满足正常的牙釉质机械性能和棱镜图案，但是需要其他牙釉蛋白接头产品来恢复牙釉质的厚度。

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