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首页> 外文期刊>Journal of cutaneous pathology >hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue.
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hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue.

机译：hTERT在黑素细胞病变中的表达：石蜡包埋组织的免疫组织化学研究。

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BACKGROUND: Telomerase plays a role in the immortalization of cells and carcinogenesis. Previous studies have yielded conflicting results on whether human telomerase RNA (hTER) expression differs in nevi, atypical nevi and melanomas using polymerase chain reaction-based telomeric repeat amplification protocol or in situ hybridization assays. The aim of this study was to evaluate human telomerase reverse transcriptase (hTERT) staining in melanocytic lesions on paraffin-embedded tissues. METHODS: Paraffin-embedded sections from 12 acquired nevi, seven dysplastic nevi, 11 Spitz nevi, eight primary invasive melanomas, and three metastatic melanomas were studied for staining intensity (0-3+) and percentage of labeled cells with anti-hTERT. RESULTS: hTERT staining was observed in most cells (>75%), in all but three lesions, and was of greater intensity in the nucleus, especially the nucleolus, compared with the cytoplasm. Spitz nevi tended to have weaker hTERT staining (mean = 1.7) compared with acquired nevi (mean = 2.2), dysplastic nevi (mean = 2.4), primary melanomas (mean = 2.4), or metastatic melanomas (mean = 3). CONCLUSIONS: Although telomerase activity was weaker in Spitz nevi, there was overlap with other nevi and primary invasive melanomas in our small series. Thus, hTERT expression does not appear to be a reliable adjunct to the histological diagnosis of primary melanocytic lesions.
机译：背景：端粒酶在细胞永生化和癌变过程中发挥作用。使用基于聚合酶链反应的端粒重复扩增方案或原位杂交测定，关于人类端粒酶RNA（hTER）表达在痣，非典型痣和黑色素瘤中是否存在差异，以前的研究产生了矛盾的结果。这项研究的目的是评估石蜡包埋组织的黑素细胞病变中的人类端粒酶逆转录酶（hTERT）染色。方法：研究了12例获得性痣，7例发育不良性痣，11例斯皮茨痣，8例原发性侵袭性黑色素瘤和3例转移性黑色素瘤的石蜡包埋切片的染色强度（0-3 +）和抗hTERT标记细胞的百分比。结果：除细胞质外，在大多数细胞中（> 75％），除三个病变外，均观察到hTERT染色，并且在细胞核（尤其是核仁）中的hTERT染色强度更高。与获得性痣（平均值= 2.2），增生性痣（平均值= 2.4），原发性黑色素瘤（平均值= 2.4）或转移性黑色素瘤（平均值= 3）相比，斯皮兹痣的hTERT染色倾向于较弱（平均值= 1.7）。结论：尽管在斯皮茨痣中端粒酶活性较弱，但在我们的小系列研究中与其他痣和原发性侵袭性黑色素瘤重叠。因此，hTERT表达似乎不是原发性黑素细胞病变的组织学诊断的可靠辅助。

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