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首页> 外文期刊>Journal of cutaneous pathology >C4d immunohistochemical stain is a sensitive method to confirm immunoreactant deposition in formalin-fixed paraffin-embedded tissue in bullous pemphigoid.
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C4d immunohistochemical stain is a sensitive method to confirm immunoreactant deposition in formalin-fixed paraffin-embedded tissue in bullous pemphigoid.

机译：C4d免疫组化染色是确认大疱性类天疱疮福尔马林固定石蜡包埋组织中免疫反应物沉积的灵敏方法。

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BACKGROUND: Bullous pemphigoid (BP) is characterized clinically by the onset of pruritic urticarial plaques, vesicles and bullae in a predominantly elderly population. While the diagnosis may be suspected on routine hematoxylin and eosin histology of formalin-fixed paraffin-embedded tissue, fresh-frozen tissue must be used to show the immunologic nature of the bullous process by direct immunofluorescence (DIF). The diagnosis is further confirmed and separated from epidermolysis bullosa acquisita (EBA) by subsequent serologic studies to detect antibodies directed against BP180 and BP230 antigens and characteristic antibody deposition on salt-split skin. METHODS: Using a polyclonal complement fragment 4d (C4d) antibody, we stained formalin-fixed paraffin-embedded skin biopsy specimens from cases of BP and controls. RESULTS: We showed characteristic linear basement membrane deposition of C4d in formalin-fixed paraffin-embedded tissue in seven of nine cases diagnosed as BP vs. EBA by DIF on fresh-frozen tissue. None of the four controls for which we had adequate tissue were positive. CONCLUSION: These results indicate that formalin-fixed paraffin-embedded tissue can be stained for the immunoreactant C4d to show characteristic immunoreactant deposition, potentially obviating the need for repeat biopsy for DIF and allowing clinicians to proceed to serologic confirmation of BP.
机译：背景：大疱性天疱疮（BP）的临床特征是在老年人群中出现瘙痒性荨麻疹斑块，囊泡和大疱。虽然可能会怀疑福尔马林固定石蜡包埋组织的常规苏木精和曙红组织学诊断，但必须使用新鲜冷冻的组织通过直接免疫荧光（DIF）显示大疱过程的免疫学性质。通过随后的血清学研究进一步确定该诊断，并将其与大疱表皮松解症（EBA）分开，以检测针对BP180和BP230抗原的抗体以及特征性抗体在盐裂皮肤上的沉积。方法：使用多克隆补体片段4d（C4d）抗体，对来自BP和对照病例的福尔马林固定石蜡包埋的皮肤活检标本进行染色。结果：在通过新鲜血液冷冻法经DIF诊断为BP与EBA的9例病例中，有7例在福尔马林固定石蜡包埋的组织中显示了C4d的线性基底膜沉积特征。我们有足够组织的四个对照中没有一个是阳性的。结论：这些结果表明，福尔马林固定石蜡包埋的组织可以进行免疫反应物C4d染色，以显示特征性的免疫反应物沉积，潜在地避免了对DIF进行重复活检的需要，并允许临床医生继续进行血压的血清学确认。

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