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首页> 外文期刊>Journal of drug targeting >Cytotoxicity of doxorubicin bound to poly(butyl cyanoacrylate) nanoparticles in rat glioma cell lines using different assays.
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Cytotoxicity of doxorubicin bound to poly(butyl cyanoacrylate) nanoparticles in rat glioma cell lines using different assays.

机译：使用不同的测定方法，在大鼠神经胶质瘤细胞系中与聚（氰基丙烯酸丁酯）纳米颗粒结合的阿霉素的细胞毒性。

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摘要

The cytotoxicity of doxorubicin bound to poly(butyl cyanoacrylate) nanoparticles (Dox-PBCA-NP) was investigated in the rat glioma cell lines GS-9L, F-98 and RG-2. MTT and LDH assays were used as cytotoxic assays. In general, the cytotoxicity of nanoparticle-bound doxorubicin (Dox) was enhanced compared to the free drug in solution. However, responses of the cell lines towards the drug effects were different. In the case of free Dox in solution, this difference correlated with different intracellular concentrations of Dox, which in turn, depended on the level of P-glycoprotein (P-gp) expression in these cell lines. Accordingly, the 9L gliosarcoma (GS-9L) cells, which appeared to be most resistant towards Dox, were characterized by the highest P-gp expression.Additionally, the influence of surfactants on the cytotoxic effect was investigated at different Dox concentrations. It was shown that the presence of polysorbate 80 (Tween 80) in the nanoparticle formulation significantly enhanced the cytotoxicity,whereas poloxamer 188 (Pluronic F68) and poloxamine 908 (Tetronic 908) had a negligible influence.
机译：在大鼠神经胶质瘤细胞系GS-9L，F-98和RG-2中研究了与聚氰基丙烯酸丁酯纳米颗粒（Dox-PBCA-NP）结合的阿霉素的细胞毒性。 MTT和LDH测定用作细胞毒性测定。通常，与溶液中的游离药物相比，结合纳米颗粒的阿霉素（Dox）的细胞毒性得到增强。然而，细胞系对药物作用的反应是不同的。在溶液中存在游离Dox的情况下，这种差异与细胞内Dox的浓度有关，而后者又取决于这些细胞系中P-糖蛋白（P-gp）表达的水平。因此，9L胶质肉瘤（GS-9L）细胞似乎对Dox具有最强的抵抗力，其P-gp表达最高。此外，还研究了表面活性剂对不同Dox浓度对细胞毒性作用的影响。结果表明，纳米颗粒配方中聚山梨酯80（Tween 80）的存在显着增强了细胞毒性，而泊洛沙姆188（Pluronic F68）和泊洛沙明908（Tetronic 908）的影响可忽略不计。

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