...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of drug targeting >Effect of electromagnetic field on endocytosis of cationic solid lipid nanoparticles by human brain-microvascular endothelial cells.
【24h】

Effect of electromagnetic field on endocytosis of cationic solid lipid nanoparticles by human brain-microvascular endothelial cells.

机译：电磁场对人脑微血管内皮细胞对阳离子固体脂质纳米颗粒内吞作用的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study investigates the electromagnetic field (EMF)-regulated transport of cationic solid lipid nanoparticles (CSLNs) across human brain-microvascular endothelial cells (HBMECs). The positive charge of CSLNs was from dioctadecyldimethyl ammonium bromide and stearylamine, and radiofrequency EMF was applied to HBMECs for promoting uptake of CSLNs. Immunochemical staining revealed that the expression of clathrin on the membrane of HBMECs enhanced during vesicular endocytosis of CSLNs. However, CSLNs and EMF slightly affected the expression of P-glycoprotein on the membrane of HBMECs. An exposure to EMF yielded negligible increase in the permeability of free saquinavir (SQV) across the HBMEC monolayer. Nevertheless, the permeability of SQV across the HBMEC monolayer increased about 17-fold when SQV was entrapped in CSLNs. Moreover, the permeability of SQV across the HBMEC monolayer increased about 22-fold by applying the CSLN encapsulation and EMF exposure. CSLNs and EMF could produce synergistic effect on improving the brain-targeting delivery.
机译：这项研究调查了电磁场（EMF）调控的人类固体-微血管内皮细胞（HBMEC）上的阳离子固体脂质纳米颗粒（CSLN）的运输。 CSLNs的正电荷来自二十八烷基二甲基溴化铵和硬脂胺，并且将射频EMF应用于HBMECs以促进CSLNs的吸收。免疫化学染色表明，网格蛋白在HBMECs的囊泡内吞过程中表达增强。然而，CSLN和EMF对HBMECs膜上P-糖蛋白的表达影响很小。暴露于EMF可使整个HBMEC单层的游离沙奎那韦（SQV）渗透率增加可忽略不计。然而，当SQV截留在CSLNs中时，SQV跨HBMEC单层的渗透性增加了约17倍。此外，通过应用CSLN封装和EMF暴露，SQV跨HBMEC单层的渗透性增加了约22倍。 CSLN和EMF可以在改善脑靶向递送方面产生协同作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号