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首页> 外文期刊>Journal of applied microbiology >Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods.
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Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods.

机译：直接从食品样品中直接检测肠毒素的金黄色葡萄球菌的快速，简单的DNA提取方法：PCR和LAMP方法的比较。

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Aims: The study describes the development of simple and rapid DNA extraction method in combination with loop-mediated isothermal amplification (LAMP) to detect enterotoxigenic Staphylococcus aureus in food samples. Methods and Results. In this study, isolation of genomic DNA of enterotoxigenic Staph. aureus from spiked milk, milk burfi, khoa, sugarcane juice and boiled rice was carried out by boiling the isolated sample pellets for 10 min with 1% Triton X-100. The isolated DNA was evaluated by polymerase chain reaction (PCR) and LAMP method. The LAMP was found to be 100 times more sensitive than PCR. The LAMP assay was very specific for Staph. aureus, and the presence of other contaminating bacterial DNAs and food matrix did not interfere or inhibit the LAMP assay. Conclusions. The template DNA extraction method developed in this study for food samples is simple, rapid and cost-effective. LAMP was found to be less sensitive to matrix effect of food, compared to PCR. Significance and Impact of the Study: The method is suitable for direct detection of Staph. aureus without any enrichment in contaminated food samples and hence finds its application in food safety analysis, in permutation with LAMP. copyright 2012 The Authors. Journal of Applied Microbiology copyright 2012 The Society for Applied Microbiology.
机译：目的：该研究描述了一种简单快速的DNA提取方法与环介导的等温扩增（LAMP）结合以检测食物样品中产肠毒素的金黄色葡萄球菌的方法的发展。方法和结果。在这项研究中，分离出产肠毒素的葡萄球菌的基因组DNA。通过用1％Triton X-100将分离的样品沉淀煮沸10分钟，从加标的牛奶，burbur牛奶，khoa，甘蔗汁和煮的米中提取金黄色葡萄球菌。通过聚合酶链反应（PCR）和LAMP方法评估分离的DNA。发现LAMP的灵敏度是PCR的100倍。 LAMP测定法对于Staph非常有特异性。金黄色葡萄球菌以及其他污染性细菌DNA和食物基质的存在均不会干扰或抑制LAMP分析。结论。本研究中开发的用于食品样品的模板DNA提取方法简单，快速且具有成本效益。与PCR相比，发现LAMP对食物的基质效应不那么敏感。研究的意义和影响：该方法适用于直接检测S鱼。金黄色葡萄球菌没有在受污染的食物样品中富集，因此可以将其应用于食品安全分析中，并通过LAMP进行排列。版权2012作者。应用微生物学期刊版权所有2012应用微生物学会。

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