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首页> 外文期刊>Journal of applied microbiology >The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis
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The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis

机译：变性梯度凝胶电泳和16S rDNA测序分析揭示了沼气池中细菌和古细菌群落的结构

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摘要

To identify the bacterial and archaeal composition in a mesophilic biogas digester treating pig manure and to compare the consistency of two 16S rDNA-based methods to investigate the microbial structure. Sixty-nine bacterial operational taxonomic units (OTU) and 25 archaeal OTU were identified by sequencing two 16S rDNA clone libraries. Most bacterial OTU were identified as phyla of Firmicutes (47p"2% of total clones), Bacteroides (35p"4%) and Spirochaetes (13p"2%). Methanoculleus bourgensis (29p"0%), Methanosarcina barkeri (27p"4%) and Methanospirillum hungatei (10p"8%) were the dominant methanogens. Only 9% of bacterial and 20% of archaeal OTU matched cultured isolates at a similarity index of greater-than-or-equal97%. About 78% of the dominant bacterial (with abundance >3%) and 83% of archaeal OTU were recovered from the denaturing gradient gel electrophoresis (DGGE) bands of V3 regions in 16S rDNAs. In the digester, most bacterial and archaeal species were uncultured; bacteria belonging to Firmicutes, Bacteroides and Spirochaetes seem to take charge of cellulolysis, proteolysis, acidogenesis, sulfur-reducing and homoacetogenesis; the most methanogens were typical hydrogenotrophic or hydrogenotrophic/aceticlastic; DGGE profiles reflected the dominant microbiota. This study gave a first insight of the overall microbial structure in a rural biogas digester and also indicated DGGE was useful in displaying its dominant microbiota.
机译：为了鉴定处理猪粪的中温沼气池中细菌和古细菌的成分，并比较两种基于16S rDNA的方法研究微生物结构的一致性。通过对两个16S rDNA克隆文库进行测序，鉴定出69个细菌操作分类单位（OTU）和25个古细菌OTU。大多数细菌OTU被鉴定为Firmicutes（总克隆的47p“ 2％），Bacteroides（35p” 4％）和Spirochaetes（13p“ 2％）的种群。Methanoculleus bourgensis（29p” 0％），Methanosarcina barkeri（27p“ 4） ％）和汉斯坦甲烷菌（10p“ 8％）是主要的产甲烷菌。只有9％的细菌和20％的古细菌OTU与培养的分离株相匹配，相似性指数大于或等于97％。从16S rDNA中V3区的变性梯度凝胶电泳（DGGE）带中回收了约78％的优势细菌（丰度> 3％）和83％的古细菌OTU。在沼气池中，大多数细菌和古细菌都未养殖。属于Firmicutes，Bacteroides和Spirochaetes的细菌似乎负责纤维素分解，蛋白水解，产酸，还原硫和均乙酰化。产甲烷最多的是典型的氢营养型或氢营养型/醋酸弹质。 DGGE谱反映了优势菌群。这项研究对农村沼气池中的整体微生物结构有了初步了解，并表明DGGE可用于展示其优势微生物群。

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