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首页> 外文期刊>Journal of applied microbiology >Response of Deinococcus radiodurans to low-pressure low-temperature plasma sterilization processes
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Response of Deinococcus radiodurans to low-pressure low-temperature plasma sterilization processes

机译：放射球菌对低压低温等离子体灭菌过程的响应

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Characterize the response of Deinococcus radiodurans R1 cells to low-pressure low-temperature nitrogen-oxygen microwave plasma and identify repair processes during recovery. Cells coated onto glass slides exhibited a biphasic plasma inactivation kinetics. Treatment with various plasmas and subsequent incubation in recovery medium prolonged the lag phase in a part of the survivors, during which the ability to grow on stress medium was recovered. This recovery strongly depended on transcriptional and translational processes and cell wall synthesis, as revealed by addition of specific inhibitors to the recovery medium. Genes involved in DNA repair, oxidative stress response, and cell wall synthesis were induced during recovery, as determined by quantitative RT-PCR. Damage to chromosomal DNA caused by plasma agents and repair during recovery was directly shown by quantitative PCR. Plasmas with less UV radiation emission were also effective in killing D. radiodurans cells but resulted in less DNA damage and lower induction of the investigated genes. The response of D. radiodurans to plasma indicates that DNA, proteins, and cell wall are primary targets of plasma finally leading to the cell death. Protein oxidation is more important for killing of D. radiodurans cells than of Bacillus subtilis spores. Thus, the contaminating biological material affects the plasma composition to be used for sterilization. The results in this study provide new insight into the interaction of plasma with bacterial cells. This knowledge contributes to the definition of useful parameters for novel plasma sterilization equipment to control process safety.
机译：表征辐射链球菌R1细胞对低压低温氮-氧微波等离子体的响应，并确定恢复过程中的修复过程。涂在载玻片上的细胞表现出双相等离子体失活动力学。用各种血浆处理并随后在恢复培养基中孵育延长了部分幸存者的延迟期，在此期间恢复了在压力培养基上生长的能力。这种恢复强烈依赖于转录和翻译过程以及细胞壁合成，如通过向恢复培养基中添加特异性抑制剂所揭示的那样。通过定量RT-PCR确定，在恢复过程中诱导了涉及DNA修复，氧化应激反应和细胞壁合成的基因。定量PCR直接显示血浆试剂对染色体DNA的损害以及恢复过程中的修复。具有较少紫外线辐射的血浆也可有效杀死D. radiodurans细胞，但导致较少的DNA损伤和较低的诱导诱导的研究基因。 D. radiodurans对血浆的反应表明DNA，蛋白质和细胞壁是血浆的主要靶标，最终导致细胞死亡。与枯草芽孢杆菌的孢子相比，蛋白质的氧化作用对杀死杜氏嗜热菌更重要。因此，污染的生物材料影响用于灭菌的血浆成分。这项研究的结果为血浆与细菌细胞之间的相互作用提供了新的见识。这些知识有助于定义新型等离子灭菌设备的有用参数，以控制过程安全性。

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