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首页> 外文期刊>Journal of dermatological science >Microarray analysis of microRNA expression in cutaneous squamous cell carcinoma
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Microarray analysis of microRNA expression in cutaneous squamous cell carcinoma

机译：皮肤鳞状细胞癌中microRNA表达的微阵列分析

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Background: MicroRNAs (miRNAs) are a novel class of short RNAs that are capable epigenetically regulating gene expression in eukaryotes. MicroRNAs have been shown to be dysregulated in a variety of cancers. The data on miRNA expression in cutaneous squamous cell carcinoma (cSCC) are very limited, and microarray-based miRNA expression profiles of cSCC have not yet been determined. Objective: To describe differentially expressed miRNAs in cSCC. Methods: Seven patients with cSCC were enrolled in the present study. Tumor biopsies (n= 7) were taken from the center of each tumor. Adjacent healthy skin (n= 7) was biopsied as a control (intraindividual control). miRNA expression profiles of all specimens were detected by microarray miRNA expression profiling based on miRBAse 16 scanning for 1205 potential human miRNA target sequences. The microarray results were confirmed by TaqMan quantitative real-time polymerase chain reaction (qRT-PCR). Results: Non-stringent filtering with a non-adjusted p≤ 0.05 revealed thirteen up-regulated and eighteen down-regulated miRNAs. Non-stringent filtering with a non-adjusted p≤ 0.01 revealed three up-regulated (hsa-miR-135b, hsa-miR-424 and hsa-miR-766) and six down-regulated (hsa-miR-30a *, hsa-miR-378, hsa-miR-145, hsa-miR-140-3p, hsa-miR-30a and hsa-miR-26a) miRNAs in cSCC. Conclusion: This study reveals differentially expressed miRNAs that may play a role in the molecular pathogenesis of cSCC and that are excellent candidates for further validation and functional analysis.
机译：背景：微小RNA（miRNA）是一类新型的短RNA，能够表观遗传地调控真核生物中的基因表达。 MicroRNA已显示在多种癌症中失调。关于皮肤鳞状细胞癌（cSCC）中miRNA表达的数据非常有限，并且尚未确定基于微阵列的cSCC miRNA表达谱。目的：描述cSCC中差异表达的miRNA。方法：本研究招募了7例cSCC患者。从每个肿瘤的中心进行肿瘤活检（n = 7）。活检邻近的健康皮肤（n = 7）作为对照（个体对照）。通过基于miRBAse 16扫描1205个潜在人类miRNA靶序列的微阵列miRNA表达谱检测所有标本的miRNA表达谱。通过TaqMan定量实时聚合酶链反应（qRT-PCR）证实了微阵列结果。结果：未经调整的p≤0.05的严格过滤显示13个上调的miRNA和18个下调的miRNA。未经调整的p≤0.01的非严格过滤显示三个上调（hsa-miR-135b，hsa-miR-424和hsa-miR-766）和六个下调（hsa-miR-30a *，hsa cSCC中的-miR-378，hsa-miR-145，hsa-miR-140-3p，hsa-miR-30a和hsa-miR-26a）miRNA。结论：这项研究揭示了差异表达的miRNA，它们可能在cSCC的分子发病机理中起作用，并且是进一步验证和功能分析的极佳候选者。

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