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首页> 外文期刊>Journal of dermatological science >The nuclear matrix shell proteome of human epidermis.
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The nuclear matrix shell proteome of human epidermis.

机译：人表皮的核基质壳蛋白质组。

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BACKGROUND: Proteomic approaches have identified cancer specific biomarker proteins in the nuclear matrix fraction of cancer cells. We wanted to determine whether a similar approach could be used to investigate melanoma biomarkers. OBJECTIVE: Since it was not clear that a nuclear matrix fraction could be isolated from the intact human epidermis, we first wanted to determine whether a nuclear matrix fraction could be isolated from the intact epidermis of human skin. If this was possible, we secondarily wanted to compare the proteome of cultured melanoma and carcinoma cells to that of the intact epidermis. METHODS: We applied two-dimensional electrophoresis (2DGE) and LC/MS/MS to identify proteins isolated in the nuclear matrix shell protein fraction isolated from the human epidermis and from cultured primary skin and cancer cells. RESULTS: A subcellular fractionation of intact epidermis succeeded in yielding a nuclear matrix shell which made up approximately 40% of total tissue protein. Only 5-10% of total cell protein was fractionated in the nuclear matrix shell of cultured skin cells. The nuclear matrix shell of the intact epidermis was distinguishable from cultured keratinocytes or HaCaT cells by expression of keratin 1. The nuclear matrix of the epidermis was distinguishable from melanocytes and melanoma cells by expression of vimentin in melanocyte-derived cells and by expression of desmoplakin in the intact epidermis. CONCLUSION: The nuclear matrix-intermediate filament system can be isolated from the intact human epidermis. A careful examination of the protein composition of this subcellular fraction from the epidermis and skin cancers may identify useful cancer specific biomarkers.
机译：背景：蛋白质组学方法已经在癌细胞的核基质部分中鉴定出了癌症特异性生物标志物蛋白。我们想确定是否可以使用类似的方法来调查黑色素瘤生物标志物。目的：由于尚不清楚是否可以从完整的人类表皮中分离出核基质部分，因此我们首先想确定是否可以从完整的人类皮肤表皮中分离出核基质部分。如果可能的话，我们第二想将培养的黑色素瘤和癌细胞的蛋白质组与完整表皮的蛋白质组进行比较。方法：我们应用了二维电泳（2DGE）和LC / MS / MS来鉴定分离自人表皮以及培养的原代皮肤和癌细胞的核基质壳蛋白部分中分离的蛋白。结果：完整表皮的亚细胞分级分离成功产生了核基质壳，该壳约占组织总蛋白的40％。在培养的皮肤细胞的核基质壳中仅将总细胞蛋白的5-10％分馏。通过角蛋白1的表达，可以将完整表皮的核基质壳与培养的角质形成细胞或HaCaT细胞区分开。通过在黑素细胞衍生的细胞中表达波形蛋白并通过在体外表达桥粒白蛋白，可以区分表皮的核基质与黑素细胞和黑素瘤细胞。完整的表皮。结论：可以从完整的人表皮中分离出核基质中间丝系统。仔细检查表皮和皮肤癌中该亚细胞部分的蛋白质组成，可能会发现有用的癌症特异性生物标志物。

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