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首页> 外文期刊>Journal of Electroanalytical Chemistry: An International Journal Devoted to All Aspects of Electrode Kinetics, Interfacial Structure, Properties of Electrolytes, Colloid and Biological Electrochemistry >A stable interface based on aryl diazonium salts/SWNTs modified gold electrodes for sensitive detection of hydrogen peroxide
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A stable interface based on aryl diazonium salts/SWNTs modified gold electrodes for sensitive detection of hydrogen peroxide

机译：基于芳基重氮盐/ SWNTs修饰金电极的稳定界面，用于过氧化氢的灵敏检测

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摘要

A stable and sensitive hydrogen peroxide (H_2O_2) biosensor based on aryldiazonium salt and SWNTs modified gold electrodes is reported. SWNTs were covalently anchored to the mixed monolayer of phenyl and 4-aminophenyl in molar ratio of 1:1 through aryldiazonium salt reaction to form stable C-C bonding. PEG molecules were introduced to the interface to resist non-specific protein adsorption. Covalent attachment of HRP to SWNTs allowed direct electron transfer to the redox protein with a rate constant of 28.6 ± 1.9 s~(-1), indicating a specific interaction between SWNTs and HRP. The covalently attached SWNTs facilitate the electrical coupling between protein and electrodes. The covalently immobilized HRP retained its catalytic activity by the enzyme responding to the addition of H_2O_2. The SWNTs/PEG/HRP modified sensing interface can be used for the detection of H_2O_2 in the range of 0.01-24 μM with a detection limit of 10 nM. Comparing to the sensing system in which HRP was physically adsorbed on the interface without the assembly of PEG, the performance of the SWNTs/PEG/HRP sensing interface has been significantly improved. The so fabricated biosensor exhibited high sensitivity, good reproducibility, and long-term stability, and can be used for the detection of H_2O_2 in real samples with good recovery.
机译：报道了基于芳基重氮盐和SWNTs修饰的金电极的稳定和敏感的过氧化氢（H_2O_2）生物传感器。通过芳基重氮盐反应，将SWNT以1：1的摩尔比共价锚定在苯基和4-氨基苯基的混合单层上，以形成稳定的C-C键。将PEG分子引入界面以抵抗非特异性蛋白质吸附。 HRP与SWNT的共价结合使电子直接转移至氧化还原蛋白，速率常数为28.6±1.9 s〜（-1），表明SWNT与HRP之间存在特异性相互作用。共价连接的SWNT促进蛋白质与电极之间的电耦合。共价固定的HRP通过响应H_2O_2的添加而保留了其催化活性。经SWNT / PEG / HRP修饰的传感界面可用于检测H_2O_2，范围为0.01-24μM，检测极限为10 nM。与在没有PEG组装的情况下将HRP物理吸附在界面上的传感系统相比，SWNT / PEG / HRP传感界面的性能得到了显着提高。如此制备的生物传感器具有很高的灵敏度，可重复性和长期稳定性，可用于实际样品中H_2O_2的检测，回收率高。

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