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首页> 外文期刊>Journal of dentistry >Effect of dentin depth on hybridization quality using different bonding tactics in vivo.
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Effect of dentin depth on hybridization quality using different bonding tactics in vivo.

机译：使用不同的体内结合策略，牙本质深度对杂交质量的影响。

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OBJECTIVES: Incomplete resin infiltration and polymerization of adhesive contributed to nanoleakage formation. This study tested the null hypothesis that adoption of different bonding tactics and dentine depth will not affect hybridization quality in vivo. METHODS: Class V cavities were prepared on the labial/buccal surface of monkey teeth. They were bonded by Single Bond (a two-step total-etch adhesive), Clearfil SE Bond (a two-step self-etch adhesive), or Clearfil S(3) Bond (an all-in-one self-etch adhesive). Combined nanoleakage analysis and quantitative immunolabeling evaluation were carried out in the hybrid layer formed in both cervical superficial and deep dentine. RESULTS: Single Bond showed reticular and spotted nanoleakage while Clearfil SE Bond and Clearfil S(3) Bond presented only a spotted one. While Single Bond showed increased concentration of labeling of type I collagen within the deep part of the hybrid layer, two self-etch adhesives-Clearfil SE Bond and Clearfil S(3) Bond revealed a homogeneous labeling pattern, even if the latter presented a significantly increased labeling index in deep dentine. CONCLUSIONS: Different bonding tactics showed different nanoleakage patterns and immunolabeling index, and was influenced by dentine depth at different levels in vivo.
机译：目的：不完全的树脂渗透和胶粘剂的聚合导致纳米渗漏的形成。这项研究检验了零假设，即采用不同的结合策略和牙本质深度不会影响体内杂交质量。方法：在猴齿的唇/颊表面准备V级腔。它们通过Single Bond（两步全蚀刻胶），Clearfil SE Bond（两步自蚀刻胶）或Clearfil S（3）Bond（多合一自蚀刻胶）粘结。 。在宫颈浅表和深部牙本质中形成的杂化层中进行了纳米泄漏分析和定量免疫标记评估的组合。结果：单键显示网状和斑点的纳米泄漏，而Clearfil SE键和Clearfil S（3）键仅显示一个斑点。虽然Single Bond显示出杂化层深处的I型胶原蛋白的标记浓度增加，但是两种自蚀刻粘合剂Clearfil SE Bond和Clearfil S（3）Bond显示出均匀的标记模式，即使后者表现出明显的标记性。深层牙本质的标记指数增加。结论：不同的结合策略表现出不同的纳米泄漏模式和免疫标记指数，并且在体内不同水平上受到牙本质深度的影响。

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