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首页> 外文期刊>Clinical toxicology: the official journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists >Myocardial and lung injuries induced by hydrogen sulfide and the effectiveness of oxygen therapy in rats.
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Myocardial and lung injuries induced by hydrogen sulfide and the effectiveness of oxygen therapy in rats.

机译：硫化氢引起的心肌和肺损伤以及氧气治疗对大鼠的有效性。

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OBJECTIVE: To study myocardial and lung injuries initiated by hydrogen sulfide, and evaluate the role and effectiveness of normobaric and hyperbaric oxygen (HBO) treatment in rats. METHODS: One hundred healthy male Wistar rats were randomly divided into five groups: A: Normal control group (no H2S); B: H2S-exposed group; C: H2S+33% oxygen treatment group; D: H2S+50% oxygen treatment group; E: H2S+HBO group. The rats in groups C, D and E were exposed to H2S in an exposure chamber (1 m3) and were made to inhale 300 ppm hydrogen sulfide for 60 min, and then they were subjected to normobaric or HBO therapy. Normobaric oxygen was at concentrations of 33% or 50%, HBO was for 100 min including compression and decompression; the rats in group A inhaled air under the same conditions. Blood was sampled immediately after the experiment for analysis of arterial blood gases, myocardial enzymes and cardiac troponin I. Lung was rapidly removed to be made into tissue homogenates and then cytochrome c oxidase activity was measured; myocardial and lung ultrastructural changes were observed by electron microscopy. RESULTS: Arterial blood gases: partial pressure of O2 (mmHg) (Group A, 97.6 +/- 8.38; B, 76.5 +/- 6.95*; C, 83.2 +/- 2.66*; D, 86.20 +/- 10.75*; E, 93.50 +/- 4.97: *p < 0.01 compared to group A) was significantly lower than that in group in all but HBO rats. For myocardial enzymes and cardiac troponin I every parameter in groups B and C was significantly higher than that in group A (p<0.01),with no difference in D and E. Cytochrome c oxidase activity (u/mg) of lung tissue was reduced compared to group A after all treatments (A, 1.76 +/- 0.02; B, 0.36 +/- 0.04; C, 0.50 +/- 0.12; D, 0.56 +/- 0.07; E, 0.68 +/- 0.05 (A vs. B p < 0.01; B vs. C,D,E p < 0.05 or p < 0.01), with a graded effect of oxygen dose in C, D and E. Pathological changes: (1) Myocardium - Mitochondrial swelling and autolysis with blurred or broken cristae was observed in the myocardium of H2S-exposed group; in group E, mitochondrial structure was basically normal, and clear cristae were found. (2) Lung tissue - In H2S-exposed group, alveolar epithelial cells disappeared, vacuolization of the organelle occurred, nuclear membrane was irregular and marginal condensation of heterochromatin was present; nucleus showed relatively normal morphology in group E, although some vacuoles still persisted within them. CONCLUSIONS: HBO therapy can effectively improve arterial oxygen partial pressure, and significantly reduce myocardial damage, as well as potentially relieve lung injury in this model. Further work in humans appears warranted.
机译：目的：研究硫化氢引发的心肌和肺损伤，并评价常压和高压氧（HBO）在大鼠中的作用和有效性。方法：将一百只健康的雄性Wistar大鼠随机分为五组：A：正常对照组（无H2S）; A：正常对照组（无H2S）; A：正常对照组（无H2S）。 B：暴露于H 2 S的基团; C：H 2 S + 33％氧气治疗组; D：H 2 S + 50％氧气治疗组; E：H2S + HBO组。 C，D和E组的大鼠在暴露室（1 m3）中暴露于H2S，并吸入300 ppm硫化氢60分钟，然后进行常压或HBO治疗。常压氧气浓度为33％或50％，HBO持续100分钟（包括压缩和减压）; A组大鼠在相同条件下吸入空气。实验结束后立即取血进行动脉血气，心肌酶和心肌肌钙蛋白I的分析。迅速去除肺组织匀浆，测定细胞色素c氧化酶活性。通过电子显微镜观察心肌和肺的超微结构变化。结果：动脉血气：O2分压（mmHg）（A组，97.6 +/- 8.38; B，76.5 +/- 6.95 *; C，83.2 +/- 2.66 *; D，86.20 +/- 10.75 *; E，93.50 +/- 4.97：与A）组相比，* p <0.01显着低于除HBO大鼠以外的所有组。对于心肌酶和心肌肌钙蛋白I，B组和C组的每个参数均显着高于A组（p <0.01），D和E组无差异。肺组织中细胞色素c氧化酶活性（u / mg）降低与所有治疗后的A组相比（A，1.76 +/- 0.02; B，0.36 +/- 0.04; C，0.50 +/- 0.12; D，0.56 +/- 0.07; E，0.68 +/- 0.05（A vs 。B p <0.01; B vs. C，D，E p <0.05或p <0.01），在C，D和E中有氧剂量的分级影响。病理变化：（1）心肌-线粒体肿胀和自溶硫化氢暴露组心肌ista裂模糊或破裂; E组线粒体结构基本正常，cr裂清晰可见;（2）肺组织-硫化氢暴露组肺泡上皮细胞消失，空泡化发生细胞器，核膜不规则，异染色质边缘凝结; E组细胞核形态相对正常，尽管有液泡仍然保留在其中。结论：HBO疗法可有效改善动脉血氧分压，并显着减少心肌损伤，并有可能减轻该模型的肺损伤。似乎有必要在人类中开展进一步工作。

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