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首页> 外文期刊>Journal of dermatological science >Irradiation of light emitting diode at 850nm inhibits T cell-induced cytokine expression.
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Irradiation of light emitting diode at 850nm inhibits T cell-induced cytokine expression.

机译：在850nm处照射发光二极管可抑制T细胞诱导的细胞因子表达。

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BACKGROUND: An anti-inflammatory effect of light obtained from light-emitting diodes (LEDs) has been discovered, however, limited ranges of wavelengths have been used and the action mechanism has been rarely demonstrated. OBJECTIVE: We sought to analyze the immunomodulatory effect of LED on Jurkat T cells and human T cells. METHODS: Jurkat T cells with/without stimulation were irradiated once or five times using seven ranges of LED wavelengths, from 415nm to 940nm. Cytotoxic effects were examined by an MTT assay. Changes in T cell-induced cytokines, including IL-2, IL-4, IL-10, IL-12 and IFN-gamma, and their upstream signaling molecules, ZAP-70 and PKCtheta, were examined by real-time PCR, ELISA, and Western blot analysis. The effect of the LED wavelength, whose effect was identified on Jurkat T cells, was also examined in human CD3+ T cells with/without stimulation and in Dermatophagoides farinae-induced atopic dermatitis (AD) NC/Nga mice. RESULTS: Lower doses of LED irradiation at 850nm inhibited T cell-derived cytokines without inducing cell death in both Jurkat T cells and human T cells. Repeated exposure resulted in a greater increase of inhibitory effects than that observed with a single exposure, and these effects were identified in the NC/Nga AD model. CONCLUSIONS: Although more remains to be clarified, these results may support the clinical application of LED for immune regulation.
机译：背景：已经发现了从发光二极管（LED）获得的光的抗炎作用，但是，已使用了有限的波长范围，并且很少证明其作用机理。目的：我们试图分析LED对Jurkat T细胞和人类T细胞的免疫调节作用。方法：使用415nm至940nm的七个LED波长范围，对有/无刺激的Jurkat T细胞进行一到五次照射。通过MTT测定法检查细胞毒性作用。通过实时PCR，ELISA检测T细胞诱导的细胞因子（包括IL-2，IL-4，IL-10，IL-12和IFN-γ）及其上游信号分子ZAP-70和PKCtheta的变化和Western blot分析。还已在有/无刺激的人CD3 + T细胞中以及在由Dermatophagoides farinae诱导的特应性皮炎（AD）NC / Nga小鼠中检查了LED波长对Jurkat T细胞有影响的作用。结果：较低剂量的850nm LED辐射抑制了Jurkat T细胞和人T细胞中T细胞衍生的细胞因子，而没有引起细胞死亡。与单次接触相比，重复接触导致的抑制作用增加更大，这些作用在NC / Nga AD模型中得到了确认。结论：尽管还有待进一步阐明，但这些结果可能支持LED在免疫调节中的临床应用。

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