• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dermatological science >Spatial and temporal analysis of skin glycation by the use of multiphoton microscopy and spectroscopy
【24h】

Spatial and temporal analysis of skin glycation by the use of multiphoton microscopy and spectroscopy

机译：使用多光子显微镜和光谱学对皮肤糖基化的时空分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Tissue glycation, the main cause of many diabetes-related complications, results in the accumulation of advanced glycation endproducts (AGE). Objectives: These AGEs are endogenous fluorophores that can serve as a viable pathological indicator for disease diagnostics. Here we explore the capabilities of multiphoton microscopy to non-invasively localize and quantify the skin glycation. Methods: In our study, multiphoton microscopy and spectroscopy were used to investigate glycation events-induced changes in the intensities of autofluorescence and second harmonic generation on ex vivo human skin. Results: Temporal and spatial dependence of degrees of glycation of the epidermis, collagen and elastin fibers of dermis were evaluated for their relevance to the changes in amplitudes of autofluorescence signals. We found that glycation drastically and linearly increases multiphoton autofluorescence intensity of epidermis and dermal collagen whereas changes in dermal elastin are moderate. We also found decrease in the level of second harmonic generation signal. Conclusion: Our study suggests that due to intrinsically weak autofluorescence the dermal collagen is the most sensitive skin tissue to be used for detecting changes in tissue glycation.
机译：背景：组织糖基化是许多糖尿病相关并发症的主要原因，导致晚期糖基化终产物（AGE）的积累。目的：这些AGEs是内源性荧光团，可作为疾病诊断的可行病理指示。在这里，我们探索了多光子显微镜非侵入性定位和量化皮肤糖基化能力。方法：在我们的研究中，使用多光子显微镜和光谱法研究糖基化事件引起的离体人体皮肤自发荧光强度和二次谐波强度的变化。结果：评估了表皮，胶原和真皮弹性蛋白糖基化程度的时空依赖性与自发荧光信号幅度变化的相关性。我们发现糖基化急剧和线性地增加了表皮和真皮胶原蛋白的多光子自发荧光强度，而真皮弹性蛋白的变化是中等的。我们还发现二次谐波产生信号的电平降低。结论：我们的研究表明，由于固有的弱自发荧光作用，真皮胶原蛋白是最敏感的皮肤组织，可用于检测组织糖基化的变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号