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首页> 外文期刊>Journal of dermatological science >Expression of antimicrobial peptides in diabetic foot ulcer.
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Expression of antimicrobial peptides in diabetic foot ulcer.

机译：糖尿病足溃疡中抗菌肽的表达。

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BACKGROUND: Foot ulcers are one of the main diabetes complications due to its high frequency and difficulty of complete healing. There are several factors that participate in diabetic ulcers development and limited information exists about the role of antimicrobial peptides (AMP) in its pathogenesis. OBJECTIVE: The aim of this study was to analyze the expression pattern of the main AMPs: Human Neutrophil Peptide (HNP)-1, Human beta-defensin (HBD)-1, HBD-2, HBD-3, HBD-4 and cathelicidin LL-37 in biopsies from diabetic foot ulcers (DFU). METHODS: 20 biopsies from DFU grade 3 according to Wagner's classification and 20 biopsies from healthy donors were obtained. Real time PCR, immunohistochemistry and primary cell cultures were performed. RESULTS: beta-Defensins were overexpressed in DFU, whereas LL-37 has low or none expression in comparison with healthy skin. When primary cell culture from these biopsies were performed and infected with Staphylococcus aureus, epidermal cell from diabetic ulcers showed lower LL-37 expression compared with cell cultures from healthy donors skin. CONCLUSION: These results suggest that though most AMPs are expressed in DFU, this production is not appropriate to promote wound healing and contain secondary infections.
机译：背景：足溃疡由于其高发和难以完全治愈而成为糖尿病的主要并发症之一。有多种因素参与糖尿病性溃疡的发展，关于抗菌肽（AMP）在其发病机理中的作用的信息有限。目的：本研究旨在分析主要AMPs的表达模式：人中性粒细胞肽（HNP）-1，人β-防御素（HBD）-1，HBD-2，HBD-3，HBD-4和cathelicidin糖尿病足溃疡（DFU）活检中的LL-37。方法：根据Wagner的分类，从DFU 3级的20份活检和20份健康的捐献者获得的活检。进行实时PCR，免疫组织化学和原代细胞培养。结果：β-防御素在DFU中过表达，而LL-37与健康皮肤相比表达低或无。当从这些活检组织进行原代细胞培养并感染金黄色葡萄球菌时，与健康供体皮肤的细胞培养相比，糖尿病溃疡的表皮细胞显示出较低的LL-37表达。结论：这些结果表明，尽管大多数AMPs在DFU中表达，但这种产生并不适合促进伤口愈合并包含继发感染。

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