...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dermatological science >Differential expression of BAFF and its receptors in discoid lupus erythematosus patients
【24h】

Differential expression of BAFF and its receptors in discoid lupus erythematosus patients

机译：盘状红斑狼疮患者BAFF及其受体的差异表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: B-cell activating factor of the TNF family (BAFF) promotes the maturation and survival of B cells. Because BAFF levels are elevated in systemic lupus erythematosus (SLE) patients, BAFF has been the target of emerging therapies for SLE, such as belimumab. Levels of BAFF and its receptors in discoid lupus erythematosus (DLE) patients are unknown. Objective: To compare skin and blood mRNA and protein levels of BAFF and its receptors BAFF-R, TACI, and BCMA in DLE subjects with (DLE. +/SLE. + (N= 28)) and without SLE (DLE. +/SLE- (N= 35)), psoriasis subjects (N= 11), and normal subjects (N= 42). Methods: We used quantitative real-time PCR to measure blood and skin BAFF, BAFF-R, TACI, and BCMA mRNA, sandwich ELISAs to measure sera BAFF, and immunohistochemistry to evaluate BAFF and BAFF-R skin protein expression. Results: BAFF mRNA and protein levels were highest in DLE+/SLE+blood, followed by DLE+/SLE-, psoriasis, and normal blood. BAFF protein also correlated with anti-nuclear antibodies, and autoantibodies against double-stranded DNA, single-stranded DNA, and ribonucleoprotein, and Systemic Lupus Erythematosus Disease Activity Index scores in DLE patients. While showing no difference between DLE+/SLE+ and DLE+/SLE- skin, BAFF and its receptors mRNA were up-regulated in DLE skin vs. normal and psoriasis skin. DLE skin had higher percentages of BAFF-R+ inflammatory cells, likely T cells and macrophages, than psoriasis and normal skin. Conclusions: BAFF may be a serologic marker of systemic disease in DLE patients. BAFF and its receptors are elevated in DLE skin, suggesting that targeted therapies against these proteins could treat refractory DLE patients.
机译：背景：TNF家族的B细胞活化因子（BAFF）促进B细胞的成熟和存活。由于系统性红斑狼疮（SLE）患者的BAFF水平升高，因此BAFF一直是新兴的SLE治疗方法（例如贝利木单抗）的目标。盘状红斑狼疮（DLE）患者的BAFF及其受体水平尚不清楚。目的：比较患有（DLE。+ / SLE。+（N = 28））和不患有SLE（DLE。+ /）的DLE受试者的皮肤和血液BAFF及其受体BAFF-R，TACI和BCMA的mRNA和蛋白质水平。 SLE-（N = 35）），牛皮癣受试者（N = 11）和正常受试者（N = 42）。方法：我们使用定量实时PCR检测血液和皮肤BAFF，BAFF-R，TACI和BCMA mRNA，使用夹心ELISA检测血清BAFF，并使用免疫组织化学评估BAFF和BAFF-R皮肤蛋白表达。结果：BAFF mRNA和蛋白质水平在DLE + / SLE +血液中最高，其次是DLE + / SLE-，牛皮癣和正常血液。 BAFF蛋白还与DLE患者的抗核抗体，针对双链DNA，单链DNA和核糖核蛋白的自身抗体以及系统性红斑狼疮疾病活动指数相关。尽管在DLE + / SLE +和DLE + / SLE-皮肤之间没有差异，但是BAFF及其受体mRNA在DLE皮肤，正常皮肤和牛皮癣皮肤中均被上调。与牛皮癣和正常皮肤相比，DLE皮肤的BAFF-R +炎性细胞（可能是T细胞和巨噬细胞）的百分比更高。结论：BAFF可能是DLE患者全身疾病的血清学标志。 BAFF及其受体在DLE皮肤中升高，表明针对这些蛋白质的靶向疗法可以治疗难治性DLE患者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号