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首页> 外文期刊>Journal of dermatological science >Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes.
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Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes.

机译：S100A8在X射线照射的角质形成细胞中的抗凋亡作用。

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BACKGROUND: Ionizing radiation is used to treat a lot of cancers, however, it also produced unwanted side effect on normal tissues, such as radiodermatitis. We previously established an animal model for radiodermatitis, and identified many of radiation-induced genes by cDNA microarray. Of the candidates, we chose S100A8 gene for a further study. OBJECTIVE: The aim of this study is to investigate the functional role of S100A8 in X-ray irradiated keratinocytes. METHODS: RT-PCR and immunohistochemistry were performed to demonstrate the S100A8 induction by X-ray irradiation. HaCaT keratinocytes were transduced with the recombinant adenovirus expressing GFP-S100A8, and then effects on cell cycle and apoptosis were analyzed using flow cytometry and Western blot. RESULTS: X-ray irradiation markedly induced S100A8 expression in the hyperplastic epidermis of mouse. Overexpression of S100A8 by adenoviral transduction led to the enhancement of cell proliferation in the absence and/or presence of X-ray irradiation, as compared with Ad/GFP control group. Furthermore, overexpression of S100A8 significantly protected the X-ray-induced apoptosis. CONCLUSION: These results suggest that S100A8 have an anti-apoptotic role in X-ray irradiated keratinocytes.
机译：背景：电离辐射用于治疗许多癌症，但是，它也会对正常组织产生有害的副作用，例如放射性皮炎。我们先前建立了放射性皮肤炎的动物模型，并通过cDNA微阵列鉴定了许多辐射诱导的基因。在候选人中，我们选择了S100A8基因进行进一步研究。目的：研究S100A8在X射线照射的角质形成细胞中的功能。方法：采用RT-PCR和免疫组化方法，通过X射线照射证明S100A8的诱导。用表达GFP-S100A8的重组腺病毒转导HaCaT角质形成细胞，然后使用流式细胞仪和Western blot分析其对细胞周期和凋亡的影响。结果：X射线照射能明显诱导小鼠增生表皮中S100A8的表达。与Ad / GFP对照组相比，在不存在和/或存在X射线的情况下，腺病毒转导引起的S100A8的过表达导致细胞增殖的增强。此外，S100A8的过表达显着保护了X射线诱导的细胞凋亡。结论：这些结果表明S100A8在X射线照射的角质形成细胞中具有抗凋亡作用。

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