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首页> 外文期刊>Biomolecules & therapeutics >Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth
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Effects of Chenopodium album Linne on Gastritis and Gastric Cancer Cell Growth

机译：藜属藜对胃炎和胃癌细胞生长的影响

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In our previous study, we investigated Chenopodium album Linne (CAL) ethanol extract and its fractions on anti-gastritic actions using the HCI/ethanol and indomethacin induced gastric lesion model and Helicobacter pylori (H. pylori). Based on the results, butanol fraction was most effective among fractions obtained from CAL. This study aims to elucidate the mechanisms of butanol fraction, and betaine as a constituent of the butanol fraction, on gastritis and anti-gastric cancer cell growth. First, we examined antioxidant properties using hydrogen peroxide and superoxide radical, and we found that butanol fraction and betaine may be good antioxidants. Second, cytotoxicity was assessed by measuring cell viability and 4,6-diamidino-2-phenylinodole dihydrochlo-ride (DAPI) staining of human gastric cancer cells (AGS cells). We also examined the relationship between the cytotoxicity and intracellular Ca~(2+) signaling mechanism. The butanol fraction demonstrated cell viability 71.49% at the concentration of 100 mugl ml and increased intracellular Ca~(2+) concentration in a dose dependent manner. Finally, we observed the mucus content as a defensive factor and gastric secretion as an aggressive factor, and found that the mucus content noticeably increased when treated with butanol fraction and betaine and gastric secretion decreased when treated with betaine in vivo study. From these results, we suggest that CAL butanol fraction and betaine may have protective effects on gastritis.
机译：在我们之前的研究中，我们使用盐酸/乙醇和吲哚美辛诱导的胃部病变模型和幽门螺杆菌（H. pylori），研究了白藜（CAL）乙醇提取物及其馏分对抗胃酸作用的作用。根据结果​​，在从CAL获得的馏分中，丁醇馏分最有效。这项研究旨在阐明丁醇级分和甜菜碱作为丁醇级分的组成部分对胃炎和抗胃癌细胞生长的机制。首先，我们使用过氧化氢和超氧自由基检查了抗氧化性能，发现丁醇级分和甜菜碱可能是良好的抗氧化剂。其次，通过测量人胃癌细胞（AGS细胞）的细胞活力和4，6-二mid基-2-苯并二氮杂二氢氯化物（DAPI）染色来评估细胞毒性。我们还研究了细胞毒性与细胞内Ca〜（2+）信号传导机制之间的关系。丁醇级分在浓度为100毫升时显示出71.49％的细胞活力，并且细胞内Ca〜（2+）浓度呈剂量依赖性。最后，我们在体内研究中观察到粘液含量是防御因子，胃分泌物是攻击性因子，发现当用丁醇级分处理时，粘液含量显着增加，而甜菜碱处理时，甜菜碱降低了胃分泌物。从这些结果，我们建议CAL丁醇部分和甜菜碱可能对胃炎具有保护作用。

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