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首页> 外文期刊>Biochimica et Biophysica Acta. General Subjects >Enzymatic properties of endo-β-N-acetylglucosaminidases from developing tomato fruits and soybean seeds: substrate specificity of plant origin endoglycosidase
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Enzymatic properties of endo-β-N-acetylglucosaminidases from developing tomato fruits and soybean seeds: substrate specificity of plant origin endoglycosidase

机译：发育中的番茄果实和大豆种子中内切β-N-乙酰氨基葡萄糖苷酶的酶学性质：植物来源内切糖苷酶的底物特异性

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摘要

Substrate specificity and some other enzymatic properties of partial purified endo-β-N-acetylglucosaminidases (endo-β-GlcNAc-ase) from developing soybean seeds (Glycine max, Endo-GM) and developing tomato fruits (Lycopersicum esculentum. Endo-LE) were studied. The substrate specificity of these two endoglycosidases was explored and compared with regard to various pyridylaminated N-glycans derived from some naturally occurring glycoproteins. For Endo-GM and Endo-LE, several high mannose-type sugar chains bearing α1-2 mannosyl residue(s), Man_(9-6)GlcNAc_2-PA (PA is pyridylamino (80-100% relative hydrolysis), were most favored substrates followed by Man_5GlcNAc_2-PA (32% for Endo-LE, 43% for Endo-GM), a typical hybrid-type structure (GlcNAc_1Man_5GlcNAc_2-PA; 34% for Endo-LE, 37% for Endo-GM), and then the common core pentasaccharide of N-glycan (Man_3GlcNAc_2-PA; 9% for Endo-GM and 16% for Endo-LE). On the contrary, both Endo-GM and Endo-LE could barely hydrolyze the xylose-containing N-glycans (Man_3Xyl_1GlcNAc_2-PA, Man_3Fuc_1Xyl_1GlcNAc_2-PA) found ubiquitously in plant cells. The molecular mass of these two endoglycosidases was approximately 62 kDa by gel filtration and both Endo-GM and Endo-LE showed maximal activities for Man_6GlcNAc_2-PA in a weak acidic region (pH 6.0-6.5).
机译：发育中的大豆种子（Glycine max，Endo-GM）和发育中的番茄果实（Lycopersicum esculentum。Endo-LE）的部分纯化的内源β-N-乙酰氨基葡萄糖苷酶（内源-β-GlcNAc-酶）的底物特异性和其他一些酶学特性被研究了。探索了这两种内切糖苷酶的底物特异性，并与衍生自某些天然存在的糖蛋白的各种吡啶化的N-聚糖进行了比较。对于Endo-GM和Endo-LE，大多数带有α1-2甘露糖基残基的高甘露糖型糖链，Man_（9-6）GlcNAc_2-PA（PA是吡啶基氨基（相对水解为80-100％））首选的底物是Man_5GlcNAc_2-PA（Endo-LE为32％，Endo-GM为43％），典型的杂合型结构（GlcNAc_1Man_5GlcNAc_2-PA; Endo-LE为34％，Endo-GM为37％），以及然后是N-聚糖的常见核心五糖（Man_3GlcNAc_2-PA; Endo-GM为9％，Endo-LE为16％），相反，Endo-GM和Endo-LE都几乎不能水解含木糖的N-普遍存在于植物细胞中的聚糖（Man_3Xyl_1GlcNAc_2-PA，Man_3Fuc_1Xyl_1GlcNAc_2-PA），这两种内切糖苷酶的分子质量约为62 kDa，经凝胶过滤，Endo-GM和Endo-LE在弱酸性条件下均表现出最大的Man_6GlcNAc_2-PA活性。区域（pH 6.0-6.5）。

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