...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of environmental monitoring: JEM >Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles
【24h】

Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles

机译：磁性发光纳米颗粒对微生物四环素抗性的定量基因监测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A magnetic/luminescent nanoparticles (MLNPs) based DNA hybridization method was developed for quantitative monitoring of antibiotic resistance genes and gene-expression in environmental samples. Manipulation of magnetic field enabled the separation of the MLNPs-DNA hybrids from the solution and the fluorescence of MLNPs normalized the quantity of target DNA. In our newly developed MLNPs-DNA assay, linear standard curves (R~2 = 0.99) of target gene was determined with the detection limit of 620 gene copies. The potential risk of increased bacterial antibiotic resistance was assessed by quantitative monitoring of tetracycline resistance (i.e., tetQ gene) in wastewater microcosms. The gene abundance and its expression showed a significant increase of tetQ gene copies with the addition of tetracycline, triclosan (TCS), or triclocarban (TCC). A real-time PCR assay was employed to verify the quantification capability of the MLNPs-DNA assay and accordingly both assays have shown strong correlation (R~2 = 0.93). This non-PCR based MLNPs-DNA assay has demonstrated its potential for gene quantification via a rapid, simple, and high throughput platform and its novel use of internal calibration standards.
机译：开发了一种基于磁性/发光纳米粒子（MLNPs）的DNA杂交方法，用于定量监测抗生素抗性基因和环境样品中的基因表达。磁场的操纵使从溶液中分离出MLNPs-DNA杂种成为可能，而MLNPs的荧光使靶DNA的量正常化。在我们最新开发的MLNPs-DNA分析中，目标基因的线性标准曲线（R〜2 = 0.99）被确定为620个基因拷贝的检测限。通过定量监测废水微观世界中四环素耐药性（即tetQ基因）来评估增加细菌抗生素耐药性的潜在风险。通过添加四环素，三氯生（TCS）或三氯卡班（TCC），该基因的丰度及其表达显示出tetQ基因拷贝的显着增加。实时PCR测定法被用于验证MLNPs-DNA测定法的定量能力，因此两种测定法均显示出强相关性（R〜2 = 0.93）。这种基于非PCR的MLNPs-DNA测定法已经证明了其通过快速，简单和高通量平台进行基因定量的潜力，以及其内部校准标准品的新颖用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号