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首页> 外文期刊>Journal of experimental & clinical cancer research : >The effects of locally injected granulocyte macrophage-colony stimulating factor on the healing of intraoperatively irradiated intestinal anastomoses in rats.
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The effects of locally injected granulocyte macrophage-colony stimulating factor on the healing of intraoperatively irradiated intestinal anastomoses in rats.

机译：局部注射的粒细胞巨噬细胞集落刺激因子对大鼠术中辐照肠吻合口愈合的影响。

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Intraoperative irradiation is used to reduce the number of local recurrences and to increase disease free survival in the treatment of intestinal malignancies. Irradiation for the local control of tumours diminishes the wound healing in the intestine as in any other tissues. For many surgeons, it seems too risky to make resection and anastomosis in an irradiated intestine. Granulocyte Macrophage-Colony Stimulating Factor (Gm-csf) had been successfully used in chronic and incisional dermal wounds. In this study, we evaluated the effect of locally applied Gm-csf on intraoperatively irradiated rat small intestinal anastomosis. 160 male Sprague-Dawley rats were randomized into 4 groups. In group 1 (control), ileal resection was made (1 cm. in length) and anastomosis was performed. In group 2, ileal resection and anastomosis were performed and 50 microgr. rHu Gm-csf was injected subserosally in the perianastomotic area. In group 3, intraoperatively 2000 cGy irradiation was applied to the intestine followingileal resection and anastomosis. In group 4, ileal resection and anastomosis were performed, and 50 microgr. rHu Gm-csf was injected subserosally in the perianastomotic area, then intraoperatively 2000 cGy irradiation was applied to the intestine. On the 3rd and 7th days, relaparotomies were made in order to measure the bursting pressures of the anastomotic segments. The measurement of hydroxyproline levels were evaluated to determine the amount of anastomotic collagen. Histopathological evaluations were also performed. The bursting pressure values in gm-csf given groups were significantly higher than their control groups. The hydroxyproline content of group 4 was significantly higher than group 3 on the 3rd day. In conclusion, these data indicate that local injection of Gm-csf improves the wound healing of intraoperatively irradiated bowel anastomosis.
机译：术中放疗可用于减少局部复发的次数，并提高治疗肠道恶性肿瘤的无病生存率。像其他任何组织一样，对肿瘤进行局部控制的辐射会减少肠道中的伤口愈合。对于许多外科医生来说，在经辐照的肠道中进行切除和吻合术似乎太冒险了。粒细胞巨噬细胞集落刺激因子（Gm-csf）已成功用于慢性和切开性皮肤伤口。在这项研究中，我们评估了局部应用Gm-csf对术中辐照大鼠小肠吻合的作用。将160只雄性Sprague-Dawley大鼠随机分为4组。在第1组（对照组）中，进行回肠切除术（长度为1厘米）并进行吻合术。在第2组中，进行回肠切除和吻合术并进行50 microgr。将rHu Gm-csf皮下注射到肛周切除区域。在第3组中，术中对肠回肠切除和吻合术进行2000 cGy照射。在第4组中，进行回肠切除和吻合术，并进行50 microgr。将rHu Gm-csf皮下注射到肛门周围的病灶区域，然后术中对肠进行2000 cGy照射。在第3天和第7天，进行再次手术，以测量吻合段的破裂压力。评估羟脯氨酸水平的测定以确定吻合胶原的量。还进行了组织病理学评估。给定组中gm-csf的破裂压力值显着高于对照组。在第3天，第4组的羟脯氨酸含量显着高于第3组。总之，这些数据表明局部注射Gm-csf可以改善术中照射肠吻合的伤口愈合。

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