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首页> 外文期刊>Journal of Experimental Botany >Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools
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Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools

机译：番茄中肉桂肉桂酰辅酶A还原酶的下调诱导可溶性酚库的急剧变化

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摘要

Health-beneficial properties of many secondary plant metabolites have created much interest into the control of their biosynthesis in crop species. Phenolic compounds, including flavonoids, hydroxycinnamates, and tannins, make up an important group of such phytonutrients. They are formed via the phenylpropanoid pathway and share common precursors with lignin, an insoluble cell wall-associated polymer. In this study, the aim was to reduce lignin biosynthesis so as to enhance the availability of these precursors and, thereby, stimulate the production of soluble, potentially health-promoting, phenolic compounds in tomato (Solanum lycopersicum L.). First two tomato genes encoding cinnamoyl-CoA reductase (CCR), a key enzyme in the formation of lignin monomers, were identified and characterized. Transgenic plants exhibiting a reduced lignin content were subsequently obtained through an RNAi strategy targeting one of these genes. As anticipated, the total level of soluble phenolics was higher in stems and leaves of the transformants as compared with control plants. This was correlated with an increased antioxidant capacity of the corresponding plant extracts. Analysis of the soluble phenolic fraction by HPLC-MS revealed that vegetative organs of CCR down-regulated plants contained higher amounts of chlorogenic acid and rutin, and accumulated new metabolites undetectable in the wild type, such as N-caffeoyl putrescine and kaempferol rutinoside. In fruits, CCR down-regulation triggered the moderate accumulation of two new compounds in the flesh, but the total phenolic content was not affected. Although the prospects of exploiting such a strategy for crop improvement are limited, the results provide further insight into the control of the phenylpropanoid pathway in the Solanaceae.
机译：许多次生植物代谢产物的有益健康的特性引起了人们对控制其在作物物种中生物合成的兴趣。酚类化合物，包括类黄酮，羟基肉桂酸酯和丹宁酸，是这类植物营养素的重要组成部分。它们通过苯丙烷途径形成，并与木质素（一种不溶的细胞壁相关聚合物）共有共同的前体。在这项研究中，目的是减少木质素的生物合成，从而提高这些前体的利用率，从而刺激番茄（茄属植物番茄）中可溶性，可能促进健康的酚类化合物的产生。首先鉴定并鉴定了编码肉桂酸辅酶A还原酶（CCR）（木质素单体形成中的关键酶）的两个番茄基因。随后通过靶向这些基因之一的RNAi策略获得了表现出降低的木质素含量的转基因植物。如所预期的，与对照植物相比，转化体的茎和叶中可溶性酚类的总水平更高。这与相应植物提取物的抗氧化能力增强相关。通过HPLC-MS分析可溶性酚类成分后发现，CCR下调植物的营养器官中含有较高量的绿原酸和芦丁，并积累了野生型中无法检测到的新代谢产物，例如N-咖啡酰腐胺和山奈酚芸豆苷。在水果中，CCR的下调触发了果肉中两种新化合物的适度积累，但总酚含量并未受到影响。尽管采用这种策略改善作物的前景有限，但结果为深入了解茄科中的苯丙烷途径提供了进一步的见识。

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