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首页> 外文期刊>Journal of Experimental Botany >A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris)
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A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris)

机译：根特异的bZIP转录因子对三元豆（Phaseolus acutifolius）和普通豆（P. vulgaris）的水分亏缺胁迫有响应

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摘要

Root cDNA libraries were differentially screened to isolate water deficit-responsive transcripts in the relatively drought-resistant plant tepary bean (Phaseolus acutifolius). A novel root-specific, water deficit-responsive transcript was identified and predicted to encode a bZIP transcription factor. The orthologous form of this gene was isolated from the drought-sensitive P. vulgaris and the patterns of expression of these genes compared. These genes have predicted amino acid sequences in the bZIP domain that are 64% similar to a soybean bZIP protein. There were three amino acid differences between the P. acutifolius bZIP and the P. vulgaris gene product. Both species transcribed this gene in a root-specific and water deficit-responsive manner. The cell-specific pattern of expression for the gene was determined using in situ hybridization and immunolocalization. Two tissues in the root accumulated the protein: epidermis and phloem. The nuclear localization of this protein was determined by electron microscopy. The bZIP protein accumulated in the nuclei of both the epidermal cell and the vascular cell in response to water deficit stress in both species in a similar manner.
机译：差异筛选根cDNA文库以分离相对抗旱的植物三生豆（Phaseolus acutifolius）中的缺水响应转录本。鉴定了一种新颖的根特异性，水分亏缺反应性转录本，并预测其编码bZIP转录因子。该基因的直系同源形式是从对干旱敏感的寻常型假单胞菌中分离出来的，并比较了这些基因的表达模式。这些基因在bZIP域中预测的氨基酸序列与大豆bZIP蛋白的相似度为64％。在P. acutifolius bZIP和P. vulgaris基因产物之间存在三个氨基酸差异。两种物种均以根特异性和缺水响应方式转录该基因。使用原位杂交和免疫定位确定基因表达的细胞特异性模式。根中的两个组织积累了蛋白质：表皮和韧皮部。通过电子显微镜确定该蛋白的核定位。响应于缺水胁迫，两种物种中的bZIP蛋白以相似的方式积聚在表皮细胞和血管细胞的细胞核中。

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