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首页> 外文期刊>Journal of Experimental Botany >The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development
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The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development

机译：甘蓝型油菜中的半乳糖醛酸酶基因BcMF2与花序发育有关

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摘要

Brassica campestris Male Fertility 2 (BcMF2) is a putative polygalacturonase (PG) gene previously isolated from the flower bud of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis). This gene was found to be expressed specifically in tapetum and pollen after the tetrad stage of anther development. Antisense RNA technology was used to study the function of BcMF2 in Chinese cabbage. Scanning and transmission electron microscopy revealed that there were deformities in the transgenic mature pollen grains such as abnormal location of germinal furrows. In addition, the homogeneous pectic exintine layer facing the exterior seemed to be overdeveloped and predominantly occupied the intine, thus reversing the normal proportional distribution of the internal endintine layer and the external exintine layer. Since it is a continuation of the intine layer, the pollen tube wall could not grow normally. This resulted in the formation of a balloon-like swelling structure in the pollen tube tip in nearly 80% of the transgenic pollen grains. Premature degradation of tapetum was also found in these transgenic plants, which displayed decreased expression of the BcMF2 gene. BcMF2 might therefore encode a new PG with an important role in pollen wall development, possibly via regulation of pectin's dynamic metabolism.
机译：甘蓝型油菜雄性育性2（BcMF2）是一种推定的聚半乳糖醛酸酶（PG）基因，先前从大白菜（甘蓝型油菜Makino，S。B. rapa ssp.chinensis）的花蕾中分离出来。发现该基因在花药发育的第四个阶段后在绒毡层和花粉中特异性表达。利用反义RNA技术研究了BcMF2在大白菜中的功能。扫描和透射电镜观察发现，转基因成熟花粉粒中存在畸形，例如生发沟的位置异常。此外，面向外部的均质果胶外泌素层似乎过度发育，并主要占据了该内因素，从而逆转了内部内泌素层和外外泌素层的正比例分布。由于是intine层的延续，因此花粉管壁无法正常生长。这导致在近80％的转基因花粉粒中，花粉管尖端形成了气球状的膨胀结构。在这些转基因植物中还发现绒毡层过早降解，显示出BcMF2基因表达降低。因此，BcMF2可能编码一种在花粉壁发育中起重要作用的新PG，可能是通过调节果胶的动态代谢来实现的。

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