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首页> 外文期刊>Journal of Experimental Botany >Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet.
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Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet.

机译：三种羽扇豆种的发育种子中脂质和蛋白质的积累：羽扇豆，羽扇豆和甜羽扇豆。

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A comparative study was carried out on the dynamics of lipid accumulation in developing seeds of three lupine species. Lupine seeds differ in lipid content; yellow lupine (Lupinus luteus L.) seeds contain about 6%, white lupine (Lupinus albus L.) 7-14%, and Andean lupine (Lupinus mutabilis Sweet) about 20% of lipids by dry mass. Cotyledons from developing seeds were isolated and cultured in vitro for 96 h on Heller medium with 60 mM sucrose (+S) or without sucrose (-S). Each medium was additionally enriched with 35 mM asparagine or 35 mM NaNO. Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid. Experiments with [1-p#tC]acetate and [2-p#tC]acetate showed that the decrease in lipid accumulation in developing lupine seeds resulted from exhaustion of lipid precursors rather than from degradation or modification of the enzymatic apparatus. The carbon atom from the C-1 position of acetate was liberated mainly as CO, whereas the carbon atom from the C-2 position was preferentially used in anabolic pathways. The dominant phospholipid in the investigated lupine seed storage organs was phosphatidylcholine. The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids. The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.
机译：对三种羽扇豆种的发育种子中脂质积累的动力学进行了比较研究。羽扇豆种子的脂质含量不同。黄色羽扇豆（Lupinus luteus L.）种子按干重计含有约6％的脂质，白色羽扇豆（Lupinus albus L.）7-14％和安第斯羽扇豆（Lupinus mutabilis Sweet）的脂质约20％。分离来自发育中种子的子叶，并在含有60 mM蔗糖（+ S）或不含蔗糖（-S）的Heller培养基上体外培养96 h。每种培养基还另外富含35 mM天冬酰胺或35 mM NaNO。天冬酰胺导致蛋白质积累增加，同时降低了脂质含量，但是硝酸盐增加了蛋白质和脂质的积累。用[1-p＃tC]乙酸盐和[2-p＃tC]乙酸盐进行的实验表明，羽扇豆种子发育过程中脂质积累的减少是由脂质前体的耗尽而不是酶设备的降解或修饰引起的。来自乙酸酯的C-1位的碳原子主要作为CO释放，而来自C-2位的碳原子优先用于合成代谢途径。在研究的羽扇豆种子贮藏器官中，主要的磷脂是磷脂酰胆碱。黄羽扇豆子叶中的主要脂肪酸为亚油酸，白羽扇豆中的主要脂肪酸为油酸，而安第斯羽扇豆中的主要脂肪酸为亚油酸和油酸。讨论了羽扇豆子叶发育过程中硝酸盐刺激脂质刺激和蛋白质积累与无机氮形式引起的糖酵解提高碳通量之间的关系。

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