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首页> 外文期刊>Journal of Experimental Botany >Jasmonates and its mimics differentially elicit systemic defence responses in Nicotiana attenuata
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Jasmonates and its mimics differentially elicit systemic defence responses in Nicotiana attenuata

机译：茉莉花及其模拟物在烟草中差异诱导系统防御反应

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摘要

Coronalon (6-ethyl indanoyl isoleucine), a synthetic jasmonate mimic, is known to regulate levels of transcripts and secondary metabolites that are commonly elicited by methyl jasmonate (MeJA) in a variety of plants. The ability of coronalon and its derivative (In-L-Ile-Me) to elicit MeJA-activated transcriptional and defence responses [nicotine and trypsin proteinase inhibitors (TPIs)] was compared in treated and systemic untreated tissues of wild-type (WT) and NaLOX3-silenced Nicotiana attenuata plants which are unable to activate either local or systemic defence responses. Coronalon and its derivative significantly regulated 71% and 86% of genes up-regulated by MeJA and 53% and 66% of the genes down-regulated by MeJA in the treated leaves, but only 3% and 7% of all regulated genes in untreated, but phylotactically connected, leaves of WT plants. Consistent with their ability to elicit transcriptional responses in treated tissues, coronalon and In-L-Ile-Me increased nicotine and TPIs when applied to the tissues in which these metabolites are produced, namely roots and leaves. However, treating roots elicited TPI activity in leaves in both WT and NaLOX3-silenced plants, suggesting that mimics can be transported apoplastically from roots to leaves in the xylem. This response was lower in NaLOX3-silenced plants, suggesting that the ability of coronalon and In-L-Ile-Me to elicit TPI responses in leaves after root treatments requires intact jasmonic acid (JA) signalling. Treating leaves did not elicit detectable changes in endogenous JA levels but did decrease free salicylic acid contents. It is concluded that coronalon and In-L-Ile-Me elicit jasmonate responses in treated tissues and could be valuable tools for dissecting local and systemic jasmonate signalling networks in plants.
机译：已知的合成茉莉花酸酯模拟物Coronalon（6-乙基茚二酰异亮氨酸）可调节多种植物中茉莉酸甲酯（MeJA）通常引起的转录本和次生代谢产物的水平。在已处理和未处理的野生型（WT）组织中比较了冠状细胞及其衍生物（In-L-Ile-Me）引起MeJA激活的转录和防御反应[烟碱和胰蛋白酶抑制剂（TPIs）]的能力。 NaLOX3沉默的烟草植物，不能激活局部或系统防御反应。在处理过的叶片中，Coronalon及其衍生物显着调节了由MeJA上调的基因的71％和86％，以及由MeJA下调的基因的53％和66％，但是在未处理的叶子中，分别只有3％和7％ ，但在进化上连接野生植物的叶子。与它们在处理过的组织中引起转录反应的能力一致，当将Coronalon和In-L-Ile-Me应用于产生这些代谢物的组织（即根和叶）时，烟碱和TPI会增加。但是，在WT和NaLOX3沉默的植物中，处理根部均会引起叶片中的TPI活性，这表明模拟物可以从根部到木质部中的根部进行质外性转运。在NaLOX3沉默的植物中，这种反应较低，表明根处理后冠冕和In-L-Ile-Me引发叶片中TPI反应的能力需要完整的茉莉酸（JA）信号传导。处理叶片未引起内源性JA水平的可检测变化，但确实降低了游离水杨酸含量。结论是，电晕和In-L-Ile-Me在处理过的组织中引起茉莉酸响应，并且可能是解剖植物中局部和全身茉莉酸信号网络的有价值的工具。

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