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首页> 外文期刊>Journal of Experimental Botany >The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress
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The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress

机译：磷脂酰胆碱水解磷脂酶C NPC4在拟南芥根对盐胁迫的响应中发挥作用

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摘要

Phosphatidylcholine-hydrolysing phospholipase C, also known as non-specific phospholipase C (NPC), is a new member of the plant phospholipase family that reacts to environmental stresses such as phosphate deficiency and aluminium toxicity, and has a role in root development and brassinolide signalling. Expression of NPC4, one of the six NPC genes in Arabidopsis, was highly induced by NaCl. Maximum expression was observed from 3 h to 6 h after the salt treatment and was dependent on salt concentration. Results of histochemical analysis of P-NPC4:GUS plants showed the localization of salt-induced expression in root tips. On the biochemical level, increased NPC enzyme activity, indicated by accumulation of diacylglycerol, was observed as early as after 30 min of salt treatment of Arabidopsis seedlings. Phenotype analysis of NPC4 knockout plants showed increased sensitivity to salinity as compared with wild-type plants. Under salt stress npc4 plants had shorter roots, lower fresh weight, and reduced seed germination. Expression levels of abscisic acid-related genes ABI1, ABI2, RAB18, PP2CA, and SOT12 were substantially reduced in salt-treated npc4 plants. These observations demonstrate a role for NPC4 in the response of Arabidopsis to salt stress.
机译：磷脂酰胆碱水解磷脂酶C，也称为非特异性磷脂酶C（NPC），是植物磷脂酶家族的新成员，可对诸如磷酸盐缺乏和铝毒性等环境胁迫做出反应，并在根发育和油菜素内酯信号传导中起作用。 NaCl可以高度诱导拟南芥中六个NPC基因之一NPC4的表达。在盐处理后3小时至6小时观察到最大表达，并取决于盐浓度。 P-NPC4：GUS植物的组织化学分析结果表明，盐诱导的表达在根尖中定位。在生化水平上，早在对拟南芥幼苗进行盐处理30分钟后，就观察到NPC酶活性的增加（由二酰基甘油的积累指示）。 NPC4基因敲除植物的表型分析表明，与野生型植物相比，盐度敏感性更高。在盐胁迫下，npc4植物的根部较短，鲜重较低，种子发芽减少。在盐处理的npc4植物中，脱落酸相关基因ABI1，ABI2，RAB18，PP2CA和SOT12的表达水平显着降低。这些观察结果证明了NPC4在拟南芥对盐胁迫的响应中的作用。

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