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首页> 外文期刊>Journal of Experimental Botany >Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes.
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Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes.

机译：转基因，甘露醇生产和耐盐的拟南芥的全球基因表达分析表明，非生物和生物胁迫相关基因的广泛变化。

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摘要

Mannitol is a putative osmoprotectant contributing to salt tolerance in several species. Arabidopsis plants transformed with the mannose-6-phosphate reductase (M6PR) gene from celery were dramatically more salt tolerant (at 100 mM NaCl) as exhibited by reduced salt injury, less inhibition of vegetative growth, and increased seed production relative to the wild type (WT). When treated with 200 mM NaCl, transformants produced no seeds, but did bolt, and exhibited less chlorosisecrosis and greater survival and dry weights than the WT. Without salt there were no M6PR effects on growth or phenotype, but expression levels of 2272 genes were altered. Many fewer differences (1039) were observed between M6PR and WT plants in the presence of salt, suggesting that M6PR pre-conditioned the plants to stress. Previous work suggested that mannitol is an osmoprotectant, but mannitol levels are invariably quite low, perhaps inadequate for osmoprotectant effects. In this study, transcriptome analysis reveals that the M6PR transgene activated the downstream abscisic acid (ABA) pathway by up-regulation of ABA receptor genes (PYL4, PYL5, and PYL6) and down-regulation of protein phosphatase 2C genes (ABI1 and ABI2). In the M6PR transgenic lines there were also increases in transcripts related to redox and cell wall-strengthening pathways. These data indicate that mannitol-enhanced stress tolerance is due at least in part to increased expression of a variety of stress-inducible genes.
机译：甘露醇是一种推定的渗透保护剂，有助于多种物种的耐盐性。用芹菜中的甘露糖-6-磷酸还原酶（）基因转化的拟南芥植物，其耐盐性显着提高（在100 mM NaCl时），其表现为减少的盐害减少，与野生型（WT）相比，抑制营养生长，并增加种子产量。当用200 mM NaCl处理时，与WT相比，转化子不产生种子，但产生螺栓，并且表现出更少的萎黄病/坏死，存活率和干重。不加盐对生长或表型没有 M6PR 作用，但是2272个基因的表达水平发生了变化。在盐的存在下， M6PR 和野生型植物之间观察到的差异要少得多（1039），这表明 M6PR 使植物预先适应了胁迫。先前的工作表明甘露醇是一种渗透保护剂，但是甘露醇的含量总是很低，可能不足以起到渗透保护作用。在这项研究中，转录组分析显示 M6PR 转基因通过上调ABA受体基因（ PYL4 ， PYL5 < / i>和 PYL6 ）以及蛋白磷酸酶2C基因（ ABI1 和 ABI2 ）的下调。在 M6PR 转基因品系中，与氧化还原和细胞壁强化途径有关的转录本也有所增加。这些数据表明，甘露醇增强的应激耐受性至少部分归因于多种应激诱导基因的表达增加。

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