...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >ALLENE OXIDE CYCLASE (AOC) gene family members of Arabidopsis thaliana: tissue- and organ-specific promoter activities and in vivo heteromerization.
【24h】

ALLENE OXIDE CYCLASE (AOC) gene family members of Arabidopsis thaliana: tissue- and organ-specific promoter activities and in vivo heteromerization.

机译：拟南芥的ALLENE OXIDE CYCLASE（AOC）基因家族成员：组织和器官特异性启动子活性和体内异聚。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Jasmonates are important signals in plant stress responses and plant development. An essential step in the biosynthesis of jasmonic acid (JA) is catalysed by ALLENE OXIDE CYCLASE (AOC) which establishes the naturally occurring enantiomeric structure of jasmonates. In Arabidopsis thaliana, four genes encode four functional AOC polypeptides (AOC1, AOC2, AOC3, and AOC4) raising the question of functional redundancy or diversification. Analysis of transcript accumulation revealed an organ-specific expression pattern, whereas detailed inspection of transgenic lines expressing the GUS reporter gene under the control of individual AOC promoters showed partially redundant promoter activities during development: (i) In fully developed leaves, promoter activities of AOC1, AOC2, and AOC3 appeared throughout all leaf tissue, but AOC4 promoter activity was vascular bundle-specific; (ii) only AOC3 and AOC4 showed promoter activities in roots; and (iii) partially specific promoter activities were found for AOC1 and AOC4 in flower development. In situ hybridization of flower stalks confirmed the GUS activity data. Characterization of single and double AOC loss-of-function mutants further corroborates the hypothesis of functional redundancies among individual AOCs due to a lack of phenotypes indicative of JA deficiency (e.g. male sterility). To elucidate whether redundant AOC expression might contribute to regulation on AOC activity level, protein interaction studies using bimolecular fluorescence complementation (BiFC) were performed and showed that all AOCs can interact among each other. The data suggest a putative regulatory mechanism of temporal and spatial fine-tuning in JA formation by differential expression and via possible heteromerization of the four AOCs.
机译：茉莉酸酯是植物胁迫反应和植物发育中的重要信号。茉莉酸（JA）生物合成中的重要步骤是通过异戊二烯氧化物环化酶（AOC）催化，其建立了茉莉酸酯的天然对映体结构。在拟南芥中，四个基因编码四个功能性AOC多肽（AOC1，AOC2，AOC3和AOC4），引发了功能冗余或多样化的问题。转录物积累的分析揭示了器官特异性表达模式，而在单个AOC启动子的控制下对表达GUS报告基因的转基因株系的详细检查显示了发育过程中部分冗余的启动子活性：（i）在完全发育的叶片中，AOC1的启动子活性，AOC2和AOC3出现在所有叶片组织中，但AOC4启动子活性是血管束特异性的。 （ii）仅AOC3和AOC4在根中显示启动子活性; （iii）在花朵发育过程中发现了AOC1和AOC4的部分特异性启动子活性。花茎的原位杂交证实了GUS活性数据。由于缺乏表明JA缺乏症的表型（例如雄性不育），单个和双重AOC功能丧失突变体的特征进一步证实了单个AOC之间功能冗余的假设。为了阐明是否多余的AOC表达可能有助于调节AOC的活性水平，进行了使用双分子荧光互补（BiFC）的蛋白质相互作用研究，结果表明所有AOC之间都可以相互作用。数据表明通过差异表达和可能的四个AOC异构化，JA形成中的时间和空间微调的推测调控机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号