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首页> 外文期刊>Journal of Experimental Botany >WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance
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WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance

机译：WRKY76是水稻转录阻遏物，在抗稻瘟病和抗冷胁迫中起相反的作用

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OsWRKY76 encodes a group IIa WRKY transcription factor of rice. The expression of OsWRKY76 was induced within 48 h after inoculation with rice blast fungus (Magnaporthe oryzae), and by wounding, low temperature, benzothiadiazole, and abscisic acid. Green fluorescent protein-fused OsWRKY76 localized to the nuclei in rice epidermal cells. OsWRKY76 showed sequence-specific DNA binding to the W-box element in vitro and exhibited W-box-mediated transcriptional repressor activity in cultured rice cells. Overexpression of OsWRKY76 in rice plants resulted in drastically increased susceptibility to M. oryzae, but improved tolerance to cold stress. Microarray analysis revealed that overexpression of OsWRKY76 suppresses the induction of a specific set of PR genes and of genes involved in phytoalexin synthesis after inoculation with blast fungus, consistent with the observation that the levels of phytoalexins in the transgenic rice plants remained significantly lower than those in non-transformed control plants. Furthermore, overexpression of OsWRKY76 led to the increased expression of abiotic stress-associated genes such as peroxidase and lipid metabolism genes. These results strongly suggest that OsWRKY76 plays dual and opposing roles in blast disease resistance and cold tolerance.
机译：OsWRKY76编码水稻的IIa组WRKY转录因子。稻瘟病菌（Magnaporthe oryzae）接种后48小时内，并通过创伤，低温，苯并噻二唑和脱落酸诱导OsWRKY76的表达。绿色荧光蛋白融合的OsWRKY76位于水稻表皮细胞的细胞核中。 OsWRKY76在体外显示出与W-box元件结合的序列特异性DNA，并在培养的水稻细胞中表现出W-box介导的转录阻遏物活性。水稻植物中OsWRKY76的过表达导致对米曲霉的易感性急剧增加，但对冷胁迫的耐受性提高。基因芯片分析表明，OsWRKY76的过表达抑制了接种稻瘟病菌后一组特定的PR基因和与植物抗毒素合成有关的基因的诱导，这与转基因水稻植株中植物抗毒素的水平仍然明显低于转基因水稻的观察结果一致。非转化对照植物。此外，OsWRKY76的过表达导致非生物胁迫相关基因（例如过氧化物酶和脂质代谢基因）的表达增加。这些结果强烈表明，OsWRKY76在瘟病抗性和耐寒性中起着双重和相反的作用。

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