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首页> 外文期刊>Journal of Experimental Botany >Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls
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Heterografting with nonself rootstocks induces genes involved in stress responses at the graft interface when compared with autografted controls

机译:与自体移植对照相比,非自体砧木异体移植在移植物界面上诱导参与应激反应的基因

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摘要

Although grafting is widely used in the agriculture of fruit-bearing crops, little is known about graft union formation in particular when two different species are grafted together. It is fascinating that two different plant species brought together can develop harmoniously as one organism for many decades. The objective of this study was to determine whether grafting two different grapevine genotypes alters gene expression at the graft interface in comparison to the presumably wound-like gene expression changes induced in autografts. Gene expression at the graft interface was studied 3, 7, 14, and 28 d after grafting in hetero- and autografts of grapevine (Vitis spp.). Genes differentially expressed between the hetero- and autografts during graft union formation were identified. These genes were clustered according to their expression profile over the time course. MapMan and Gene Ontology enrichment analysis revealed the coordinated upregulation of genes from numerous functional categories related to stress responses in the hetero- compared to the autografts. This indicates that heterografting with nonself rootstocks upregulates stress responses at the graft interface, potentially suggesting that the cells of the graft interface can detect the presence of a nonself grafting partner.
机译:尽管嫁接已广泛用于水果类作物的农业,但对于嫁接结合的形成知之甚少,特别是当将两种不同的物种嫁接在一起时。令人着迷的是,在一起聚集的两种不同植物物种可以作为一种生物体和谐地发展数十年。这项研究的目的是确定与自体移植诱导的伤口样基因表达变化相比,移植两种不同的葡萄基因型是否会改变移植物界面的基因表达。在异种和自种葡萄(Vitis spp。)移植后3、7、14和28 d,研究了移植物界面的基因表达。鉴定了在移植物结合形成过程中异种移植物和自体移植物之间差异表达的基因。这些基因根据它们在整个时间过程中的表达谱聚类。与自体移植相比,MapMan和Gene Ontology的富集分析揭示了与异种应激反应相关的众多功能类别的基因协同上调。这表明用非自身砧木进行异种移植会上调移植物界面处的应激反应,这可能表明移植物界面的细胞可以检测到非自身移植物伴侣的存在。

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