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首页> 外文期刊>Journal of Experimental Botany >TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets
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TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets

机译：TaHsfA6f是一种转录激活因子，可调节小麦（Triticum aestivum L.）中的一组热应激保护基因，包括以前未知的Hsf靶标

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摘要

Heat stress is a significant environmental factor adversely affecting crop yield. Crop adaptation to high-temperature environments requires transcriptional reprogramming of a suite of genes involved in heat stress protection. This study investigated the role of TaHsfA6f, a member of the A6 subclass of heat shock transcription factors, in the regulation of heat stress protection genes in Triticum aestivum (bread wheat), a poorly understood phenomenon in this crop species. Expression analysis showed that TaHsfA6f was expressed constitutively in green organs but was markedly up-regulated during heat stress. Overexpression of TaHsfA6f in transgenic wheat using a drought-inducible promoter resulted in up-regulation of heat shock proteins (HSPs) and a number of other heat stress protection genes that included some previously unknown Hsf target genes such as Golgi anti-apoptotic protein (GAAP) and the large isoform of Rubisco activase. Transgenic wheat plants overexpressing TaHsfA6f showed improved thermotolerance. Transactivation assays showed that TaHsfA6f activated the expression of reporter genes driven by the promoters of several HSP genes (TaHSP16.8, TaHSP17, TaHSP17.3, and TaHSP90.1-A1) as well as TaGAAP and TaRof1 (a co-chaperone) under non-stress conditions. DNA binding analysis revealed the presence of high-affinity TaHsfA6f-binding heat shock element-like motifs in the promoters of these six genes. Promoter truncation and mutagenesis analyses identified TaHsfA6f-binding elements that were responsible for transactivation of TaHSP90.1-A1 and TaGAAP by TaHsfA6f. These data suggest that TaHsfA6f is a transcriptional activator that directly regulates TaHSP, TaGAAP, and TaRof1 genes in wheat and its gene regulatory network has a positive impact on thermotolerance.
机译：热胁迫是对作物产量产生不利影响的重要环境因素。作物适应高温环境需要对涉及热应激保护的一系列基因进行转录重编程。这项研究调查了TaHsfA6f（热休克转录因子A6亚型的成员）在调节小麦（面包小麦）中热应激保护基因的作用，这种现象在该作物物种中鲜为人知。表达分析表明，TaHsfA6f在绿色器官中组成性表达，但在热应激期间明显上调。使用干旱诱导型启动子在转基因小麦中过表达TaHsfA6f导致热休克蛋白（HSP）和许多其他热应激保护基因的上调，这些基因包括一些以前未知的Hsf靶基因，例如高尔基抗凋亡蛋白（GAAP） ）和Rubisco活化酶的大型同工型。过表达TaHsfA6f的转基因小麦植物显示出更高的耐热性。反式激活试验表明TaHsfA6f在以下条件下激活了几种HSP基因（TaHSP16.8，TaHSP17，TaHSP17.3和TaHSP90.1-A1）以及TaGAAP和TaRof1（共同伴侣）的启动子驱动的报告基因的表达。非压力条件。 DNA结合分析显示这六个基因的启动子中存在高亲和力的TaHsfA6f结合热激元件样基序。启动子截短和诱变分析确定了TaHsfA6f结合元件，这些元件负责TaHsfA6f对TaHSP90.1-A1和TaGAAP的反式激活。这些数据表明TaHsfA6f是直接调节小麦中TaHSP，TaGAAP和TaRof1基因的转录激活因子，其基因调控网络对耐热性具有积极影响。

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