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首页> 外文期刊>Journal of Experimental Botany >Ubiquitin ligase EL5 maintains the viability of root meristems by influencing cytokinin-mediated nitrogen effects in rice
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Ubiquitin ligase EL5 maintains the viability of root meristems by influencing cytokinin-mediated nitrogen effects in rice

机译：泛素连接酶EL5通过影响水稻中细胞分裂素介导的氮效应来维持根分生组织的活力

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摘要

Root formation is dependent on meristematic activity and is influenced by nitrogen supply. We have previously shown that ubiquitin ligase, EL5, in rice (Oryza sativa) is involved in the maintenance of root meristematic viability. When mutant EL5 protein is overexpressed to dominantly inhibit the endogenous EL5 function in rice, primordial and meristematic necrosis ia observed. Here, we analysed the cause of root cell death in transgenic rice plants (mEL5) overexpressing EL5V162A, which encodes a partly inactive ubiquitin ligase. The mEL5 mutants showed increased sensitivity to nitrogen that was reflected in the inhibition of root formation. Treatment of mEL5 with nitrate or nitrite caused meristematic cell death accompanied by browning. Transcriptome profiling of whole roots exhibited overlaps between nitrite-responsive genes in non-transgenic (NT) rice plants and genes with altered basal expression levels in mEL5. Phytohormone profiling of whole roots revealed that nitrite treatment increased cytokinin levels, but mEL5 constitutively contained more cytokinin than NT plants and showed increased sensitivity to exogenous cytokinin. More superoxide was detected in mEL5 roots after treatment with nitrite or cytokinin, and treatment with an inhibitor of superoxide production prevented mEL5 roots from both nitrite- and cytokinin-induced meristematic cell death. These results indicate a nitrogen-triggered pathway that leads to changes in root formation through the production of cytokinin and superoxide, on which EL5 acts to prevent meristematic cell death.
机译：根的形成取决于分生组织的活动，并受氮供应的影响。以前我们已经表明，水稻（水稻）中的泛素连接酶EL5与维持根分生组织的活力有关。当突变型EL5蛋白过表达以显着抑制水稻的内源性EL5功能时，观察到原始和分生性坏死。在这里，我们分析了过表达EL5V162A的转基因水稻植株（mEL5）中根细胞死亡的原因，EL5V162A编码部分无活性的泛素连接酶。 mEL5突变体显示出对氮的敏感性增加，这反映在对根形成的抑制中。用硝酸盐或亚硝酸盐处理mEL5会导致分生组织细胞死亡，并伴有褐变。整个根的转录组分析显示非转基因（NT）水稻植物中亚硝酸盐响应基因与mEL5中基础表达水平改变的基因重叠。全根的植物激素谱分析表明，亚硝酸盐处理增加了细胞分裂素水平，但mEL5组成性地比NT植物含有更多的细胞分裂素，并显示出对外源细胞分裂素的敏感性增加。用亚硝酸盐或细胞分裂素处理后，在mEL5根中检测到更多的超氧化物，并且用超氧化物生成抑制剂处理可防止mEL5根同时受亚硝酸盐和细胞分裂素诱导的分生组织细胞死亡。这些结果表明氮触发的途径可通过细胞分裂素和超氧化物的产生而导致根形成的变化，EL5可在该途径上防止分生细胞死亡。

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