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首页> 外文期刊>Journal of Experimental Botany >Arabidopsis membrane-associated acyl-CoA-binding protein ACBP1 is involved in stem cuticle formation
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Arabidopsis membrane-associated acyl-CoA-binding protein ACBP1 is involved in stem cuticle formation

机译：拟南芥膜相关的酰基辅酶A结合蛋白ACBP1参与茎表皮的形成

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摘要

The membrane-anchored Arabidopsis thaliana ACYL-COA-BINDING PROTEIN1 (AtACBP1) plays important roles in embryogenesis and abiotic stress responses, and interacts with long-chain (LC) acyl-CoA esters. Here, AtACBP1 function in stem cuticle formation was investigated. Transgenic Arabidopsis transformed with an AtACBP1pro::GUS construct revealed beta-glucuronidase (GUS) expression on the stem (but not leaf) surface, suggesting a specific role in stem cuticle formation. Isothermal titration calorimetry results revealed that (His)(6)-tagged recombinant AtACBP1 interacts with LC acyl-CoA esters (18:1-, 18:2-, and 18:3-CoAs) and very-long-chain (VLC) acyl-CoA esters (24:0-, 25: 0-, and 26:0-CoAs). VLC fatty acids have been previously demonstrated to act as precursors in wax biosynthesis. Gas chromatography (GC)-flame ionization detector (FID) and GC-mass spectrometry (MS) analyses revealed that an acbp1 mutant showed a reduction in stem and leaf cuticular wax and stem cutin monomer composition in comparison with the wild type (Col-0). Consequently, the acbp1 mutant showed fewer wax crystals on the stem surface in scanning electron microscopy and an irregular stem cuticle layer in transmission electron microscopy in comparison with the wild type. Also, the mutant stems consistently showed a decline in expression of cuticular wax and cutin biosynthetic genes in comparison with the wild type, and the mutant leaves were more susceptible to infection by the necrotrophic pathogen Botrytis cinerea. Taken together, these findings suggest that AtACBP1 participates in Arabidopsis stem cuticle formation by trafficking VLC acyl-CoAs.
机译：膜固定拟南芥的酰基-COA结合蛋白1（AtACBP1）在胚胎发生和非生物胁迫反应中起重要作用，并与长链（LC）酰基-CoA酯相互作用。在这里，研究了AtACBP1在茎角质层形成中的功能。用AtACBP1pro :: GUS构建体转化的转基因拟南芥揭示了茎（而不是叶）表面上的β-葡萄糖醛酸苷酶（GUS）表达，表明其在茎角质层形成中具有特定作用。等温滴定量热法结果显示（His）（6）标记的重组AtACBP1与LC酰基辅酶A酯（18：1、18：2-和18：3-CoA）和超长链（VLC）相互作用酰基-CoA酯（24：0-，25：0-和26：0-CoA）。先前已证明VLC脂肪酸可作为蜡生物合成的前体。气相色谱（GC）-火焰电离检测器（FID）和GC-质谱（MS）分析显示，与野生型（Col-0）相比，acbp1突变体显示茎和叶表皮蜡和茎角质单体的组成减少）。因此，与野生型相比，acbp1突变体在扫描电子显微镜下的茎表面蜡晶体更少，而在透射电子显微镜下的茎表皮层不规则。而且，与野生型相比，突变体茎始终显示出表皮蜡和角质生物合成基因的表达下降，并且突变体叶片更易受到坏死性病原体灰葡萄孢的感染。在一起，这些发现表明AtACBP1通过贩运VLC酰基辅酶A参与拟南芥茎角质层的形成。

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