...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce
【24h】

WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce

机译：与WUSCHEL相关的HOMEOBOX 8/9对于裸子植物挪威云杉的正确胚胎构图非常重要

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Proper embryo development is crucial as that is when the primary body axes are established. In Arabidopsis, AtWOX8 and AtWOX9, members of the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, are critical for embryo development. In Norway spruce, PaWOX8/9, which is expressed in embryos, is the homologue of AtWOX8 and AtWOX9. In this work, it is shown that the transcript abundance of PaWOX8/9 is high during early and late embryogeny and that it decreases when the maturation phase starts. To address the function of PaWOX8/9 during embryo development, RNAi lines were established to down-regulate the transcript level of PaWOX8/9, using both constitutive and inducible promoters. Embryos in the PaWOX8/9 RNAi lines show an aberrant morphology caused by disturbed orientation of the cell division plane at the basal part of the embryonal mass during early and late embryogeny. In addition, the transcript level of several key cell-cycle-regulating genes, for example, PaE2FAB-LIKE and PaCYCLIN B-LIKE, are affected in the PaWOX8/9 RNAi lines. Taken together, our results suggest that PaWOX8/9 may perform an evolutionarily conserved function as a regulator of the establishment of the apical-basal embryo pattern.
机译：正确的胚胎发育至关重要，因为这是建立主要身体轴的时间。在拟南芥中，与WUSCHEL相关的HOMEOBOX（WOX）基因家族的成员AtWOX8和AtWOX9对胚胎发育至关重要。在挪威的云杉中，在胚胎中表达的PaWOX8 / 9是AtWOX8和AtWOX9的同源物。在这项工作中，表明PaWOX8 / 9的转录本丰度在早期和晚期胚胎形成过程中较高，并且在成熟阶段开始时降低。为了解决PaWOX8 / 9在胚胎发育过程中的功能，建立了RNAi系以使用组成型和诱导型启动子下调PaWOX8 / 9的转录水平。 PaWOX8 / 9 RNAi系中的胚胎显示出异常的形态，这是由于在早期和晚期胚胎发生过程中细胞分裂平面在胚团基部的取向受到干扰而引起的。此外，PaWOX8 / 9 RNAi系会影响几个关键的细胞周期调控基因的转录水平，例如PaE2FAB-LIKE和PaCYCLIN B-LIKE。两者合计，我们的结果表明PaWOX8 / 9可能执行进化上保守的功能，作为建立根尖基胚模式的调节剂。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号